National Institutes of Health, NIAID, Rocky Mountain Laboratories, 903 South 4th Street, Hamilton, MT 59840

66 -- FURNISH,INSTALL AND PROVIDE TRAINING FOR A PROTEIN PURIFICATION SYSTEM. DUE 082399 POC Leona Padrotti,PA,(406) 363-9395/Lynda Kieres,Contracting Officer,(406) 363-9210 E-MAIL: Click here to contact the Purchasing Agent via e-mail., LPadrotti@niaid.nih.gov. 17. This notice is a combined synopsis/solicitation for commercial items prepared in accordance with the format in Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; quotes are being requested and a written solicitation will not be issued. This procurement is being issued as a request for quotation. Submit offers on RFQ # 906. The solicitation documents and incorporated provisions and clauses are those in effect through Federal Acquisition Circular 97-12. This acquisition will be processed under Simplified Acquisition Procedures (SAP) and is not a Small Business Set-Aside. The associated Standards Industrial Classification Code (SIC) for this procurement is 3826 and the small business size is 500 employees. SCHEDULE: The Rocky Mountain Laboratories, Laboratory of Human Bacterial Pathogenesis (LHBP) has a requirement for a protein purification system to purify molecules from complex starting materials derived from cell lysates, tissue homogenates, recombinant protein expression systems and other sources. The system must be able to perform separations based on a variety of sample characteristics, such as molecular weight, net surface charge, and hydrophobicity, while retaining biological activity. The system must have the capability to automate the running of large open columns, as well as a variety of gel filtration media. The system must be a two-pump gradient system that can provide a flow rate range of 0.001 to 10 ml/min and a pressure range from 0-25 Mpa (3625 psi). The two-pump gradient system must provide for dynamic mixing and provide shallow gradients for high-resolution separations of biomolecules. The two-pump and mixer configurations produce high accuracy and precision in gradient formation. The system controller must allow for the automation of the optimization of separation parameters (sample volume, flow rate, gradient slope, buffer pH). This allows a small amount of sample to be consumed in optimization of the purification scheme. In order to facilitate cleaning, storage and use of columns, the system must include two motorized valves, one for the column inlets and one for the column outlets. The controller must allow programs to be time-based, column-based, or column-volume based. The accompanying software must allow the user to enter details of the next purification at the same time the current separation is being done and simultaneously integrate and evaluate the results of earlier runs. The system controller and software must monitor three pumps, six motorized valves, a fraction collector, a dynamic gradient mixer, a chart recorder, UV monitor and a conductivity pH monitor. The system shall include a fraction collector that allows fixed volume fractionation or automatic peak fractionation. The system shall also include a 350 MHz Intel Pentium PC which has, at a minimum: 100 MHz bus speed; 512 KB cache; 6.4 GB Ultra ATA hard drive; 3.5" 1.44" floppy drive; 24x CD-ROM drive; Iomega internal ZIP100 drive (IDE version); ATI Rage PRO Turbo AGP2x enhanced graphics; 4 MB video SGRAM; ESS ES1869 embedded sound card; 1 AGP expansion slot; 2 ISA expansion slots; 5 PCI Expansion slots; 2 serial and 1 parallel ports; 104 key enhanced keyboard; PS/2 style mouse; 17" monitor; network interface card; Microsoft Windows NT 4.0 Workstation 4.0; and standard 3 year limited warranty. FOB Point shall be Destination, Hamilton, MT. Inside delivery, which shall include installation and set-up, shall be 30 days after receipt of order (ARO). Training for up to two persons shall be included at the convenience of the laboratory. Delivery location is Rocky Mountain Laboratories, 903 S. 4th Street, Hamilton, MT. The following FAR provisions and clauses apply to this acquisition: 52.212-1 Instructions to Offerors Commercial; FAR 52.212-2 Evaluation Commercial Items; FAR 52-212-4 Contract Terms and Conditions Commercial Items; FAR 52-212.5 Contract Terms and Conditions Required to Implement Statues or Executive Orders Commercial Items. The following FAR clauses, which are cited in clause 52.212-5(a) and (b) are applicable to this acquisition as follows: FAR 52.222.3, Convict Labor; FAR 52.233-3, Protest After Award; FAR 52.222-26, Equal Opportunity; FAR 52.222-35, Affirmative Action for Special Disabled and Vietnam ERA Veterans; FAR 52-222.36, Affirmative Action for Workers with Disabilities; 52.222-37, Employment Reports on Special Disabled Veterans and Veterans of the Vietnam Era; 52.225-3 Buy American Act Supplies; 52.225-21, Buy American Act North American Free Trade Agreement Implementation Act Balance of Payments Program. Offerors must include with their offer a completed copy of the provisions at FAR 52.212-3 Offerors Representations and Certifications Commercial Items. The Government intends to make a best value buy to the responsible offeror whose offer is most advantageous to the Government. Evaluation will include (i) the technical capability of the item offered to meet the agency need and (ii) price. Warranty information to include period and coverage, shall be stated. Offerors must include with their offer certification that all hardware and software offered are Y2K compliant. Offers may be mailed or faxed to the POC indicated above (Fax -- 406-363-9288). Offers must be submitted not later than 4:30 PM (MDST), 08/23/99. Copies of the above-referenced clauses are available upon request, either by telephone or fax. All responsible sources may submit an offer that will be considered by this Agency. Posted 08/06/99 (W-SN364524). (0218)

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