National Cancer Institute, Research Contracts Branch, PSAS, 6120 Executive Blvd, EPS/Room 638, Bethesda, MD 20892-7227

A -- COLLECTION OF BLOOD AND PROSTATIC TISSUE SAMPLES SOL RFQ90180 DUE 090799 POC Debbie Moore, 301-402-4509 The Division of Cancer Epidemiology and Genetics (DCEG), Environmental Epidemiology Branch, Hormonal Studies Section, National Cancer Institute (NCI) plans to procure through the University of Oklahoma, Health Science Center, Department of Urology, 920 Stanton L. Young Blvd., GRW-3150, Oklahoma City, Oklahoma 73104 the collection of blood and prostatic tissue samples. NCI is conducting a methodologic study to correlate tissue levels of hormones with serum levels in order to clarify whether circulating levels of hormones reflect hormonal status in the prostate gland. A total of 60 men undergoing prostatectomy shall be recruited into the study for tissue and blood collection. Six 50-200mg pieces of frozen tissue should be collected from each of the three sites in the prostate gland, including the transitional, central, and peripheral zones. In total, 18 pieces of tissue and 30ml of blood samples will be collected from each of 60 study subjects. A total of 1,080 prostatic samples and 60 30ml of blood samples will be collected. The contractor shall: 1) Collect fresh prostatic tissue before the blue ink is applied to the removed prostrate gland. (This is critical since blue ink may interfere with the assays of tissue hormones); 2) Collect 18 pieces of fresh tissue from 3 different zones of the prostate gland to provide enough samples from the same study subject; 3) Possess ability and shall measure hormone levels in the single cell from collected tissue. (Dr. Hemstreet of the University of Oklahoma developed the Quantitative Fluorescence-image Analysis (QFIA) technique to measure hormone content in a single cell); 4) Process the blood within two hours after collection, and store the processed blood and tissue at -70 degrees C. The features described herein will enable NCI to assess intra-prostatic variations in tissue hormones. In addition, the QFIA technique allows NCI to quantify hormone contents in individual cells in relation to their morphology or relative to other cells. Quantification of single cells may provide insights into the relationship between hormones and prostate cancer. The University of Oklahoma is the only source known to NCI that can meet all the above requirements. This is not a request for competitive quotation. However, if any interested party believes it can meet the above requirement, it may submit a statement of capabilities. The statement of capabilities and any other information furnished must be in writing and must contain material in sufficient detail to allow NCI to determine if the party can perform this requirement. Capability statements must be received in the contracting office by 3:00 p.m. EST, on September 7, 1999. If you have any questions, please contact Debbie Moore on (301) 402-4509. A determination by the Government not to compete this proposed requirement based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. Posted 08/19/99 (W-SN370145). (0231)

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