National Cancer Institute, Research Contracts Branch, PSAS, 6120 Executive Blvd, EPS/Room 638, Bethesda, MD 20892-7227

66 -- FLUORESCENT IMAGING SYSTEM SOL NCI-RFQ-90226-NR DUE 092019 POC Marsha Gorham, Purchasing Agent & Todd Cole, Contracting Officer The National Cancer Institute (NCI), Division of Basic Science (DBS), Laboratory of Cellular Carcinogenesis and Tumor Promotion (LCCTP), intends to procure on a sole-source basis, a Fluorescent Imaging System, from Intracellular Imaging, Inc.. The NCI investigator requires an instrument that can be used in the analysis of the regulation of vanilloid receptors. This receptor for vanilloids acts as a calcium channel. Analysis has shown that it can cause calcium to flow into the cytoplasm from two locations. The assay currently in use for calcium response can only monitor calcium flow from one of these two locations (the requested system shall monitor and measure calcium flow from both locations). The rate of calcium response is also an important parameter to on-going research (the system shall measure the rates of response on a sub-second time scale (4 to 5 measurements per second). The system shall monitor single cells and shall allow the visualization of both calcium levels and GFP (green fluorescent protein) tagged protein kinase C. Additionally, a system is required to monitor the relationship between intracellular calcium levels and protein kinase C localization. The fluorescence Imaging System shall include the following: Imaging workstation; excitation illumination source; computer controlled filter changer; low light level CCD camera; InCyt IM2 image acquisition and analysis software; system electronics and cabling; optional Olympus CK 40 inverted microscope with shutter wheel, GFP/FURA-2 dichroic cube and filter set; 20X Fluorphase, 10X UV Oil, Cooled CCD Camera Substitution, Computer Upgrades 256 RAM, readwrite, CD, 25.5GB, installation, training and one year warranty. The NCI investigator believes that the aforementioned instrument and organization is the only source that can completely satisfy their requirements. The Contractor shall provide a Year 2000 Warranty for each hardware, software, and firmware product delivered. The award will be in the aggregate for all items listed herein. This is not a request for competitive quotation, however, if any interested party believes it can meet the above requirement, they may submit a statement of capabilities. Information furnished must be in writing and must contain sufficient detail, to allow NCI to determine if the party can meet the requirement. Capability statements must be received in the Contracts Office by 2:00 PM EST on September 20, 1999. If you have any questions, please contact Marsha Gorham, Purchasing Agent on (301) 496-8607. A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. Posted 09/01/99 (W-SN375169). (0244)

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