National Institute of Standards & Technology, Acquisition & Assistance Div.,100 Bureau Drive Stop 3572, Bldg. 301, Rm B117, Gaithersburg, MD 20899-3572

A -- ASSEMBLY & TESTING A FLOW CYTOMETER SOL 53SBNB967090 DUE 091399 POC Contracting Officer, Patrick Staines, (301) 975-6335 WEB: NIST Contracts Homepage, http://www.nist.gov/admin/od/contract/contract.htm. E-MAIL: NIST Contracts Office, Contract@nist.gov. The National Institute of Standards and Technology (NIST) intends to negotiate on a sole source basis with Howard Shapiro, MD, PC for the assembly and testing of a Flow Cytometer as specified herein: 1. Signal processing and data collection: Digital signal processing of pulses, minimum of 8 samples per pulse at 14 bit resolution, minimum 800K samples per second, at least a 4-decade nominal dynamic range, fast serial or equivalent digital interface between DSP and PC: PC-based, list mode files complying with FCS 2.0 standard 32-bit analysis software, digital logarithmic transformation, analysis of subsections of linear data from histograms of at least 65,536 channels, digital compensation, NT operating system 2. Fluorescence and scattering detectors : Detection hardware for scattered light: Forward scatter: photodiode, avalanche photodiode(APD), or photomultiplier (PMT); able to resolve 1 mm plastic particles. Orthogonal scatter: APD or PMT High voltage(HV) supplies for APD/PMT to be provided; PMT supplies preferably integral with Cockcroft-Walton voltage multipliers. DC coupled preamplifiers for APD or PMT provided. Detection hardware for fluorescence: Red sensitive PMT, HV and preamplifier provided, Signal diagnostics, Reference electronic pulse input, Provision for LED test pulse, Provision for extra fluorescence channel 3. Assembly and testing of cytometer at NIST, Model 532 argon ion laser, 488nm, 20 mW (supplied by NIST) Optical table(4 ft by 4 ft, inch holes, supplied by NIST) Flow cell: Rectangular or square cross section quartz, collection optics of at least 0.68 N.A. for orthogonal fluorescence and scatter, at least 0.10 N.A. for forward scatter. Flow control; Pressure-driven sheath, Syringe pump feed for samples with provision for computer control of pump. 4. Technical support for a period of one year. The cytometer will be used for research purposes, therefore it has to be modular and expandable. Dr. Shapiro is the only responsible source due to the flexibility and expertise to design and assemble the cytometer with unique capabilities as required for NIST research. No solicitation will be issued. NIST anticipates awarding a firm fixed priced purchase order on or before 30 Sept 1999. If you should have any questions regarding this announcement please contact Patrick Staines at (301) 975-6335 or via email at Patrick.Staines@nist.gov. Posted 09/01/99 (W-SN375032). (0244)

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