National Institute of Diabetes, Digestive & Kidney Diseases (NIDDK), Acquisition Management Branch, Natcher Building, 45 Center Dr., MSC 6600, Room 6AN32, Bethesda, Maryland 20892-6600

99 -- SERVICES OF A VENDOR FOR THE PRODUCTION OF KNOCKOUT TRANSGENIC MICE SOL NIH-NIDDK-99-480 DUE 092999 POC Desiree Kelly, Purchasing Agent 301-594-7731 The National Institutes of Health (NIH) National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) has a requirement to purchase the services of a vendor for the Production of Knockout Transgenic mice. This requirement will be used to produce a cre/lox dependent knockout mouse removing the O-linked GlcNAc Transferase locus. This is part of a long-term effort to characterize this locus and define its role in type II diabetes and the functioning of the islet cells of the pancreas. The government laboratory will supply the BAC (Bacterial Artificial Chromosome) clone containing the OGT gene and provide sequence and positional information regarding the locus of interest. This will included confidential sequence information and a general strategy for the production of the targeting vector. The Government will supply any information required for production of probes and other items required for the production. The vendor will be required to 1) Generally Map the OGT gene in sufficient detail to make a targeting vector 2) Prepare a targeting vector (cre/lox) which will allow gene disruption 3) Introduce the vector into recipient Embryonic Stem cells by genetic selection 4) Inject the stem cells into psuedo pregnant females for production of chimeras 5) Breed to strain insuring germline transmission of the knockout allele 6) Breed knockout strain to homogygosity 7) Provide the government with 5 breeding pairs of mice carrying the mutant allele for subsequent breeding to a targeted cre expressing mouse strain. 8) A laboratory facility that is AALAC approved or has their approved pathogen-free animal facility. It is anticipated that these steps should be completed by the vendor within 6-8 months of initial contact with the government laboratory. The Standard Industrial Classification Code is 3011 and the business size standard is 500. All responsible sources may submit a quotation, which if timely received, shall be within the Simplified Acquisition Threshold for Purchases up $100,000. It is the intent of the National Institute of Diabetes and Digestive and Kidney Disease to purchase the required laboratory services specified in the Request for Quote (RFQ). Vendors capable of furnishing the government this service should request a copy of the request for Quote form from the above contact. It is anticipated that Request for Quotation (RFQ) NIH-NIDDK-99-480 will be available fifteen (15) days after the publication date of this synopsis. Receipt of quotations will be due ten (10) days after the publication date of this synopsis. The anticipated award date is September 27, 1999. Request for copies must be in writing and may be FAX to 301-4380-4226. Quotations must be submitted to the National Institute of Diabetes and Digestive and Kidney Diseases Room: 6AN32, 45 Center Drive MSC 6600 Bethesda, MD 20892 Attention: Desiree Kelly FAX quotations must contain offerors signed by an authorized company representative. Posted 09/02/99 (W-SN375840). (0245)

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