National Cancer Institute, Research Contracts Branch, PSAS, 6120 Executive Blvd, EPS/Room 638, Bethesda, MD 20892-7227

66 -- CRYOTRANSFER SYSTEM SOL NCI-RFQ-90234-NR DUE 092319 POC Marsha Gorham, Purchasing Agent & Todd Cole, Contracting Officer (301)402-4509 The National Cancer Institute (NCI), Division of Basic Science (DBS), Laboratory of Biochemistry, intends to procure on a sole-source basis, a Cryotransfer System, from Gatan, Inc. The Cryotransfer System is required for the preparation of frozen hydrated specimens for examination by a Transmission Electron Microscope (TEM), manufactured by Philips Electronic Instrument Co. The Cryotransfer System will be used for, 1) structure determination of membrane proteins and membrane protein complexes at atomic resolution and, 2) structure determination of macromolecular assemblies from single particle imaging studies. The Cryotransfer System shall have the following features: shall allow frozen suspensions to be loaded into a TEM holder and transferred frost-free at temperatures less than minus 150 degrees C to the TEM; shall be comprised of a single tilt, liquid nitrogen cooled specimen holder and include tools for the frost-free transfer of grids from the workstation to the holder; shall allow the specimen temperature to be measured and varied between the minimum temperature and room temperature; shall provide power to a second heater used to generate the cryopump in the liquid nitrogen dewar; shall have an automatic warm-up cycle for use when removing the sample holder from the TEM and a zeolite bake-out cycle for holder maintenance, programmed into the controller; the system shall be completely compatible and work with the existing Government-owned PhilipsTEM. Shall include: the Cryotransfer System #626.DH with side entry TEM specimen holder, workskstation, specimen clamping system, specimen grid holder and, a Smart SCT cooling holder / dual heater control unit #900.60XX. The Contractor shall provide a Year 2000 Warranty for each hardware, software, and firmware product delivered. The award will be in the aggregate for all items listed herein. This is not a request for competitive quotation, however, if any interested party believes it can meet the above requirement, they may submit a statement of capabilities. Information furnished must be in writing and must contain sufficient detail, to allow NCI to determine if the party can meet the requirement. Capability statements must be received in the Contracts Office by 2:00 PM EST on September 23, 1999. If you have any questions, please contact Marsha Gorham, Purchasing Agent on (301) 402-4509. A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. Posted 09/07/99 (W-SN376839). (0250)

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