National Cancer Institute, Research Contracts Branch, PSAS, 6120 Executive Blvd, EPS/Room 638, Bethesda, MD 20892-7227

66 -- SKYVISON CYTOGENETICS WORKSTATION SOL NCI-RFQ-90243-NR DUE 092419 POC Marsha Gorham, Purchasing Agent & Todd Cole, Contracting Officer (301)402-4509 The National Cancer Institute (NCI), Division of Clinical Sciences (DCS), Laboratory of Pathology plans on procuring a SkyVision Cytogenetics Workstation with SpectraCube Spectral Imaging System from Applied Spectral Imaging, Inc., 2120 Las Palmas Drive, Suite B, Carlsbad, CA 92009. (Other Accessories to be purchased include: C-Mount Adaptor for Zeiss Axiskop 2, Universal Light Source with trigger, 150 Watt Xenon Bulb, Sky filter set, DAPI filter set for Zeiss, filter cube for Zeiss,printer and test format kits). The Laboratory of Pathology has three missions: 1) to provide state-of-the-art clinical cytogenetics services to patients evaluated and treated at NIH, 2) to perform innovative cutting edge cytogenetics research, and 3) to train students, residents, and fellows in basic principles of cytogenetics. In the research being performed, particularly on adult nonhematologic solid tumors and cell lines, the researchers are unable to completely analyze or define them because of the complexity of the chromosomal rearrangements or "cryptic" rearrangements which are not visible by G-banding techniques. Therefore, the aforementioned equipment is required for use with and assembly on an available Zeiss Axioscope 2 microscope. The system shall have the following features: 1) be capable of automatic karyotyping of human (24 colors) metaphase chromosome spreads, based upon a single hybridization with appropriate combinatorial-labeled DNA probes, and one-pass spectral readout from the specimen; 2) employ algorithms using the fluorescence spectra of the pure dyes used in DNA-probe labeling thus avoiding any prior identification of chromosomes; 3) be based upon an imaging, fourier-transform spectrometer attachable via a C-mount to the available Zeiss microscope; 4) have an optical head that shall employ a common-path interferometer that ensures the system is stable, durable and easily calibrated with any standard sourceor set of narrow band interference filters; 5) have optical path difference (OPD) for the Optical Head that is controlled by a variable speed, high accuracy, digital position-controlled qalvanometer; 6) to enable 24/21 color karyotyping, have broad spectra response from 400 nm to 1000 nm, high variable spectra resolution adjustable by the user from inside the software and spatial resolution limited only by pixel size; 7) have an interferometer that does not degrade achievable and spatial resolutions; 8) include thermoelectrically cooled, low noise, high dynamic range, progressive scan interline CCD camera with 659 x 494 pixels, stand alone camera controller and rasterizing computer board; 9) provide images that are acquired automatically in the interferometer, fourier-transformed in no longer than 2 minutes and rapidly transferred at a rate of 1 M pixel/sec or better in digital format to the RAM of the computer via direct memory access. The system software shall enable several types of interferometer/CCD calibrations, including fringe and spectral calibration, as well as optical alignment. It should provide the ability to define multiple reference spectra for storage in a reference library and/or from within an image of interest. It also should have the ability to do spectral browsing to view and display in real time spectra measured at any pixel in the image being viewed. The software shall also display images in CIE corrected RGB colors, effectively combine the G- or DAPI-banded image with multi-color spectral and karyotyping information and human chromosome ideograms, save images in more than one file format, export output spectral data to ASCII files, and do wavelength scanning, as well as spectral and spatial averaging. In addition, the user shall be able to perform basic arithmetic image transformations, to add, subtract, divide, and multiply spectral images to view the results immediately. Applied Spectral Imaging, Inc. is the only source known to the NCI Researcher that offers and interferometer-based system that uses one-pass accurate spectral readout to automatically reconstruct 24 color human karyotypes. This notice of intent is not a request for competitive quotation. However, if any interested party believes it can meet the above requirements, it may submit a statement of capabilities. The capability statement and any other furnished information must be in writing and must contain material in sufficient detail to allow NCI to determine if the party can fully meet the requirements. Capability statements must be received in the contracting office by 3:00 p.m., EST on September 24, 1999. If you have any questions, please contact Marsha Gorham, Purchasing Agent on (301)402-4509. A determination by the Government not to compete this proposed requirement based on responses to this notice is solely within the discretion of the Government. Information received shall be considered solely for the purpose of determining whether to conduct a competitive procurement. No collect calls will be accepted. Posted 09/08/99 (W-SN377567). (0251)

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