National Cancer Institute, Research Contracts Branch, PSAS, 6120 Executive Blvd, EPS/Room 638, Bethesda, MD 20892-7227

66 -- FOUR COLOR FLOW CYTOMETER SOL NCI-RFQ-90248-NR DUE 092419 POC Marsha Gorham, Purchasing Agent & Todd Cole, Contracting Officer The National Cancer Institute (NCI), Division of Clinical Sciences (DCS), Medicine Branch (MB) plans on procuring a four color flow cytometer, the FACSCalibur, from Becton Dickinson Immunocytometry Systems, 2350 Qume Drive, San Jose, CA 95131. The Medicine Branch has been involved in the development of patient-specific idiotype vaccines for human follicular lymphoma and multiple myeloma patients. The patients receive the vaccine in the Medicine Branch . The addition of a four color flow cytometer will enable the Medicine Branch to perform additional investigations, including: 1) Analysis of intracellular cytokine profiles in fresh PBMC prepared from patients under vaccine therapy during scheduled visits and in the tumor-specific T cells generated in vitro from these patients; 2) Phenotype analysis of biopsy tumor specimens of interest; 3) Phenotype analysis of ID-specific T cells generated from lymphoma and myeloma patients; 4) Mapping of antigenic epitope(s) specific for individual patient tumors; 5) Characterization of dendritic cells generated from patients/donors under the bone marrow transplantation protocol; and 6) Sorting of CD8+ T cells from fresh PBMC and tumor antigen-simulated primary PBMC cultures to establish antigen-specific CTL. The Medicine branch requires a four color flow cytometer for the above studies. Specifically, the cytometer shall have the following characteristics and features: 1) Shall enable multiparameter flow cytometry analysis of different cell populations; 2) Shall have an Argon Ion Laser and a compatible diode laser to provide measurement of four colors (four fluorescent channels); 3) Shall allow the four color measurement in addition to the forward and side scatter parameters; 4) Shall be a benchtop, modular design but adequately support dual laser operation; 5) Shall allow for maximum fluorochrome and antibody choices by providing five or more photomultiplier tubes; 6) The dual laser beams shall be alignment free with spatial and temporal separation of the beams; 7) Instrument shall insure automatic electronic separation of spectra overlap and provide accurate measurements of fluorescence; 8) The instrument flow cell shall be at least 480 by 400 microns; and 9) Shall have a Macintosh Power PC, monitor, and a laser printer. Becton Dickinson is the only source known to the NCI Researcher that can meet the above specifications. This notice of intent is not a request for competitive quotation. However, if any interested party believes it can meet the above requirement, it may submit a statement of capabilities. The capability statement and any other furnished information must be in writing and must contain material in sufficient detail to allow NCI to determine if the party can fully meet the requirements. Capability statements must be received in the contracting office by 3:00 p.m., EST on September 24, 1999. If you have any questions, please contact Debbie Moore, Purchasing Agent on (301)402-4509. A determination by the Government not to compete this proposed requirement based on responses to this notice is solely within the discretion of the Government. Information received shall be considered solely for the purpose of determining whether to conduct a competitive procurement. No collect calls will be accepted. Posted 09/08/99 (W-SN377930). (0251)

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