DHHS/PHS/FDA/Office Of Facilities, Acquisition, And Central Services, Division of Contracts and Program Management, HFA-512(COB), 5600 Fishers Lane, Rockville, MD 20857

R -- MOLECULAR MECHANISMS OF DEVELOPMENTAL TOXICITY AND ABNORMALTIES SOL 167926JP DUE 102599 POC Julie Pearson, Contract Specialist, (301)827-7155 The Food and Drug Administration (FDA) intends to award a purchase order on a sole source basis for a twelve (12) month period to Pathology Associates International, 15 Worman's Mill Court, Suite I, Frederick, Maryland 21701. Pathology Associates International will provide the following services: 1)Perform gross and histologic examination of fetal/embryonic tissue to assay segmental and other defects, 2) Analyze embryonic tissues for qualitative and quantitative changes in genetic expression and identify unique proteins that are expressed in response to physical and chemical exposure using gel electrophoresis techniques, immunochemical.immunohistochemical methodologies, etc., 3) Perform assays to identify alterations in the proliferation of cells with the presomitic mesoderm and other issues of the developing embryo following in vivo and in vitro hyperthermia treatment, 4) Analyze embryonic tissues for the specific DNA endonuclease activity that is accelerated during programmed cell death using some ofthe following technigues: endonuclease activation, gel electrophoresis for DNA fragmentation, in situ hybridization and quantitative RT-PCR, 5) Analyze embryonic tissues for identification of unique gene expression following hyperthermia using thermo-stable DNA polymerase and specific primers for a specific sequence or gene of interest (heat shock genes/proteins;homeobox genes/proteins, etc.). Pathology Associates International is currently providing FDA with highly trained and specialized personnel and the immediate access to highly specialized molecular and immunopathology technologies as well as advanced teratologic assays. Pathology Associates are uniquely capable of of fulfilling the requirements with no interruption of laboratory processes. Posted 10/14/99 (W-SN391672). (0287)

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