National Cancer Institute, Research Contracts Branch, PSAS, 6120 Executive Blvd, EPS/Room 638, Bethesda, MD 20892-7227

66 -- INSTRUMENTS & LABORATORY EQUIPMENT SOL RFQ-NCI-00012 DUE 110999 POC Cynthia Brown, Purchasing Agent, 301-402-4509, Todd Cole, Contracting Officer  The National Cancer Institute (NCI), Extracellular Matrix Pathology Section intends to procure on a sole-source basis with Amersham Pharmacia, 33148 Treasury Center, Chicago, ILL 60694 under the authority of 41.U.S.C. 253(c)(1), FAR 6.3201- to procure an AKTA Explorer 100A/FRAC-901 Fraction collector and Compaq Deskpro 450MHZ w/Zip Drive. The NCI believes that the aforementioned instrument and organization is the only source that can satisfy their requirement. The AKTA Explorer System must be able to perform the following: ability to purify biomolecules from complex starting materials derived from cell lysates, tissue homogenates, recombinant protein expression systems and other sources. The system shall be able to perform separations based on a variety of sample characteristics, such as molecular weight, net surface charge, hydrophobicity, while retaining biological activity. It shall also provide the capabilities of precess development, analysis and scale-up. imaging systems shall have the following features. Specific features required: 1)a P-901 two-pump system than can provide a flow rate range of 0.01 to 100 ml/min and a pressure range from 0-10 Mpa (1450PSI); 2)two switch valves that allow for accurate, automatic buffer preparation called BufferPrep; 3)a M-925 single chamber mixer powered and controlled from the P-901 pump. Interchangeable mixing chambers of 0.6 and 2ml.;4)ph/C-900 monitor that measures in-line conductivity, temperature and pH, to allow for auto ranging between any two ionic-strength buffers with a conductivity range of luS/cm to 999mS/cm and a PH range 0-14 with plus or minus 0.1 pH unit accuracy, temperature compensated; 5)contain two on-line air sensors to assure that air is not pumped onto the columns in the event the buffers are depleted; 6) a seven port INV-907 injection valve with load, inject and wash positions; 7)two eight port PV-908 motorized valves to accommodate up to seven columns, plus one column bypass, automated reverse-flow capability for elution of samples from affinity columns; 8)a FRAC-901 fraction collector to allow fixed volume fractionation or automatic peak fractionation; 9)the systems controller and software must be able to monitor three pumps, six motorized valves, a fraction collector, a dynamic gradient mixer, a chart recorder, UV monitor and conductivity/pH monitor. All equipment/software delivered must be Year 2000 compliant. This is not a request for competitive quotation, however, if any interested party believes it can meet the above requirement, they may submit a statement of capabilities. Information furnished must be in writing and must contain sufficient detail, to allow NCI to determine if the party can meet the requirement. Capability statements must be received in the Contracts Office by 2:00 P.M. EST on 11/9/99. If you have any questions, please contact Cynthia Brown, Purchasing Agent on (301) 402-4509. A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. Posted 10/18/99 (W-SN392540). (0291)

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