USDA, ARS, PPD, Contracts Branch, 5601 Sunnyside Avenue, Rm. 3-2126-C, Beltsville, MD 20775-5100

68 -- REAGENTS & SUPPLIES SOL RFP 02-3K06-00 DUE 021120 POC Darleen Masten, Contract Officer at (301) 504-1709 or EMAIL: dmasten@ars.usda.gov WEB: USDA, ARS Solicitation Page, http://www.ars.usda.gov/afm2/divisions/ppd/solicit.htm. E-MAIL: Contracting Officer Darleen Masten, dmasten@ars.usda.gov. The USDA, ARS, intends to purchase the following reagents/supplies for use with their Perkin Elmer Biosystems model 3700 DNA Analyzers under an indefinite delivery-indefinite quantity type contract under full and open competition as brand name or equal. The catalog numbers are Perkin Elmer Biosystem, Inc., 850 Lincoln Center Drive, Foster City, CA 94404, numbers unless otherwise noted. Note, some items do not have a catalog number. 01A, 5x Sequencing Dilution Buffer, 600 reactions; 01B, 5x Sequencing Dilution Buffer, 5400 reactions; 02, Matrix Standard Set DS-01, Sequencing; 03, Matrix Standard Set DS-30, Fragment Analysis; 04, Fluorescent-labelled Size Standard 400HD-Rox; 05, Fluorescent-labelled Size Standard 500-Rox; 06, GeneScan-400HD ROX, Catalog; 07, Genotyping Demo Kit DS-30; 08, GeneScan Install Standard DS-30; 09, Ultrapure Formamide (minimum 99.5% pure w/low water content, packed under inert gas & conductivity of -- 100uSiemens/cm or less; if preparing injection buffer); 10, Ion exchange resin (if preparing dFormamide injection buffer); 11, Na2EDTA (if preparing dFormamide injection buffer); 12, Isopropanol (HPLC grade for 3700); 13, 100% Isopropanol (anhydrous; for sequencing reaction precipitations); 14, 75% Isopropanol (2-propanol; for sequencing reaction precipitations); 15, 95% Ethanol (ACS reagent grade, non-denatured; for sequencing reaction precipitations); 16, Nitric Acid (working dilution 1N); 17, 0.2mL MicroAmp Reaction Tubes without caps (Used with Matrix standards and spatial calibration kit). 2000/Box; 18, MicroAmp 96-Well Plate; 19, Costar 96-Well Polystyrene Microtiter Plate (1 plate/ run); 20A, Caps for MicroAmp 96-Well Plate (12 caps/strip); 20B, Caps for MicroAmp 96-Well Plate (8 caps/strip); 20C, Colored Caps for MicroAmp 96-Well Plate (12 caps/strip); 20D, Colored Caps for MicroAmp 96-Well Plate (8 caps/strip); 20E, Bulkpack Caps for MicroAmp 96-Well Plate (12 caps/strip); 20F, Bulkpack Caps for MicroAmp 96-Well Plate (8 caps/strip); 20G, MicroAmp Cap Installing Tool;21, MicroAmp 384-Well PCR Plate; 22, MicroAmp Optical 96-Well Reaction; 23A, MicroAmp Full Plate Cover for 96-Well Plates; 23B, MicroAmp Full Plate Cover for 24-Well Plates; 24, PCR Microamp optical 96-Well Plate (20 plates/pkg.and 2400 caps); 25, PCR Microamp optical 384-Well Plate (250 plates/pkg. with 100 sealing tape/pkg.); 26, GeneAmp PCR System 9700 Termal Cycler, 96-Well Block, 27, Dual 384-Well GeneAmp PCR System 9700 Thermal Cycler; 28, Acrodisc In-line Filter, 02.um; 29, Adhesive-backed aluminum foil tape; 30, Array IT 96-Well Plate (for sequencing reaction column purification); 31, Array IT 384-Well Plate (for sequencing reaction column purification); 32, Array IT Clean-up Kits: 32A, For 96-Well Plate, Start-up Kit (1 x 96); 32B, For 96-Well Plate, Mini Start-up Kit (10 x 96); 32C, For 96-Well Plate, Midi Kit (50 x 96); 32D, For 96-Well Plate, Maxi Kit (100 x 96); 32E, For 384-Well Plate, Start-up Kit (1 x 384); 32F, For 384-Well Plate, Mini Start-up Kit (10 x 384); 32G, For 384-Well Plate, Midi Start-up Kit (10 x 384); 32H, For 384-Well Plate, Maxi Kit (10 x 384); 33A, Post-PCR BigDye Cleanup kit, (10 plates/kit); 33B, Post-PCR BigDye Cleanup kit, (2 plates/kit); 34, AGCT Centriflex Dye Terminator cleanup cartirdge units, 108 individual columns/unit; 35, Array-It cleanup cartridge units, 96 well block cartridges; 36, Centri-Sep 96-Well Filter Plates (for sequencing reaction column purification); 37, GeneAmp PCR System 9600 Thermal Cycler; 38, Screw-cap chemical resistant polypropylene container, 25mL size suggested (for storage of dFormamide injection buffer); 39, Eppendorf 0.2 mL; 40, Eppendorf 1.5 mL tubes; 41A, Micropipets and plugged tips; 41B, Micropipets and plugged tips; 41C, Micropipets and plugged tips; 42, Micro Amp Clear Adhesive Flims for sealing of microAmp plates for storage, 100 films/pack; and 43, Qiagen BioRobot-Qia Prep Turbo plasmid kit (4 kits/pkg.). The anticipated release date is mid January 2000. The solicitation will be available on the Internet at the following URL location in Wordperfect and ASCII: http://www.ars.usda.gov/afm2/divisions/ppd/solicit.htm. Interested parties must download SF1449, the solicitation, and all corresponding attachments. Please be advised that any subsequent amendments to this solicitation may be posted at this same web site without further notice. All offerors are responsible for the complete package and any subsequent amendments. There may be a link on this web page for interested offerors to sign up on a listbot. Offerors who have signed up on the listbot will receive E-mail notification of the solicitation posting and amendment postings. Anticipated solicitation closing date is 3:00pm, EST, on February 11, 2000. Dates are subject to change. SIC 2869. RFP 02-3K06-00. ***** Posted 12/17/99 (W-SN409530). (0351)

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