National Institute of Diabetes, Digestive & Kidney Diseases (NIDDK), Acquisition Management Branch, Natcher Building, 45 Center Dr., MSC 6600, Room 6AN32, Bethesda, Maryland 20892-6600

99 -- SERVICES CELL BIOLOGIST(FLOW CYTOMETRIST) TO CULTURE AND MAINTAIN HEMOATOPIETIC, ERYTHROID AND NEOPLASTIC CELL FOR ONE YEAR SOL NIH-NIDDK-00-531 DUE 021400 POC Desiree Kelly, Purchasing Agent, 301-594-7731 The National Institutes of Health (NIH), National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) has a requirement to purchase the services of Cell Biologist (Flow Cytometrist) to Culture and Maintain Hemoatopietic, Erythroid and Neoplastic Cell for one year. The requirements for the person are as follows: 1) High speed cell sorting using a coulter elite instrument. The candidate should be highly familiar with the instrument and be able to align lasers, repair minor hardware faults, and operate the software for data generation, retrieval, and statistical analysis. The candidate must possess expertise in high-speed sorting of human and other mammalian hematopietic cells. The candidate must have expertise in identifying erythroid cell and erythroid cell precursor populations among mixed populations of primary and neoplastic cells. 2) Staining and preparation of cells for flow cytometry. The candidate must possess expertise in staining and preparation of cellular molecules for flow cytometric detection. These include surface molecules, as well as, nucleic acids and lipid moieties. The candidate must be skilled in staining methods specifically applicable to erythroid cell subsets. 3) Culturing of primary human erythroid cells and mammalian cell lines. The candidate must be capable of media preparation and sterile techniques involved in the culture of primary human hematopoietic cells and neoplastic cells. Special expertise must include knowledge of erythroid cell culture methods. The candidate must also be aware of precautions used in the handling of samples infected with human pathogens. 4) Cell transfection with genetic materials and assessment of gene transfer efficiencies using flow cytometry. The candidate must be familiar with an able to perform gene transfer experiments using genes encoding green fluorescent protein. Expertise with flow-cytometric assessment of gene transfer is required. 5) Reagent storage and records. The candidate must be able to order and maintain the reagents necessary for the above requirements. The candidate must also be able to maintain the frozen laboratory cell stocks. The Period of Performance is for 225 days over a one year period. The Standard Industrial Classification is 9999. And the business size standard is 500. All responsible small business sources and 8(a) sources may submit a quotation, which if timely received, shall be within the Simplified Acquisition Threshold for Purchases up $100,000. It is intent of the National Institute of Diabetes and Digestive Kidney Diseases to purchase the required Cell Biologist specified in the Request for Quotation (RFQ) NIH-NIDDK-00-531 will be available fifteen (15) days after the publication date of this synopsis. Receipt of quotations will be due ten (10) days after the release of the RFQ. The anticipated award date is February 16, 2000. Request for copies must be in writing and may be FAX to 301-480-4226. Quotations must be submitted to the National Institute of Diabetes and Digestive and Kidney Disease: 6705 Rockledge Drive, Rockledge One, Suite 8048, Bethesda, MD 20817 Attention: Desiree Kelly FAX quotations must contain offerors signed by an authorized company representative. Posted 01/19/00 (W-SN417061). (0019)

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