U.S. Environmental Protection Agency, 26 W. Martin Luther King Dr., Cincinnati, OH 45268

A -- ANALYTICAL SERVICES FOR PRODUCTION OF SPORES AND DETERMINATION OF VIABLITY AND INFECTIVITY SOL RFQ-OH-00-00061 POC Kathleen Roe, phone: (513)487-2144, fax: (513)487-2109 WEB: Office of Acquisition Management -- Cincinnati Contracts, This acquisition will not appear on the web site.. E-MAIL: U. S. EPA Cincinnati Contracts Management Division, roe.kathleen@epa.gov. The U.S. EPA National Risk Management Research Laboratory intends to negotiate on a sole source basis with the University of Arizona for analytical services for the production of Encephalitizoon intestinalis spores (3 X 109) required to conduct chloramine inactivation experiments and to determine viability and infectivity assays for this organism. The contractor shall provide analytical services for 52 samples. Spores shall be provided for the samples from individual disinfection experiments and the viability of the spores shall be determined for each sample. Each experiment shall consist of four exposure times based upon exposing the spores to different levels of chloramine disinfectant at various exposure times (conducted in duplicate). Spores shall be shipped to EPA by overnight carrier at various time intervals specified by the Agency. After exposure to the disinfectant, the spores will be shipped back to the contractor for viability determinations. These determinations shall be made based upon growth in tissue culture media (RPM1-7) in accordance with prescribed protocol. The contractor shall provide a report which describes the viability results for each sample within four weeks after receipt of the spores. EPA research indicates that the University of Arizona is the only source capable of providing the numbers of E. intestinalis spores required for this type of disinfection procedure. However, any organization or firm believing itself capable of meeting the requirements may submit technical documentation to demonstrate its potential of complying with the statement of work. Only those organizations presenting such documentation will be considered. Otherwise, EPA will proceed to negotiate with Arizona University on a sole source basis under authority of FAR Part 6.302. Information received will normally be considered solely for the purpose of determining whether to conduct a competitive procurement. A determination not to compete this acquisition based on responses to this notice is solely within the discretion of the Government. This notice of intent is not a request for quotations, however, all quotations received within 15 days after electronic publication of this notice will be considered by the Government. This acquisition will be processed under FAR Part 13, Simplified Acquisition Procedures. Posted 02/15/00 (W-SN425385). (0046)

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