National Cancer Institute, Research Contracts Branch, PSAS, 6120 Executive Blvd, EPS/Room 638, Bethesda, MD 20892-7227

66 -- UPGRADE AXIOVERT 100 TO EXISTING ATTOFLUOR SOL NCI-RFQ-00072-NV DUE 032300 POC Debbie Moore, Purchasing Agent 301-402-4509, Gail Zois, Contract Specialist 301-402-4509 The National Cancer Institute (NCI) on behalf of the National Institute of Neurological Disorders and Stroke (NINDS) plans to procure an Attofluor ratiovision digital ratio imaging system upgrade with real time video-based ratioing for an existing Zeiss inverted Axiovert 100 System from Carl Zeiss, Inc., Medical Consumer Department, Carl Zeiss Drive, Thornwood, NY 10594. NINDS requires the above upgrade in order to study the following: 1) the regulatory elements in the OT and VP genes that are responsible for cell-specific expression; 2) electrical activity, neurotransmitter, neurohormone and growth factor receptors as they pertain to regulation of gene expression; 3) posttranslational processing enzymes and mechanisms involved in the production of biologically active peptides from precursor proteins; 4) calcium dependent excitation-secretion coupling mechanisms in the nerve terminals of neurohypophysis; and 5) biophysical and related molecular properties of peptidergic neurons. The Attofluor ratiovision digital ratio imaging system upgrade is completely compatible to the existing Zeiss inverted Axiovert 100 System from Carl Zeiss. In addition, the upgrade will provide the following functionality required by the researcher: 1) simultaneous video acquisition, analysis and viewing of ration, single excitation and transmitted light images; 2) calibrated excitation and emission ratioing with simultaneous background subtraction, shading correction, and exclusion for up to 99 regions; 3) simultaneous or sequential multi-dye capability; 4) integrated atto data analysis; 5) flexible image viewing from experimental data sets; 6) an acquisition rate of 30 full field image (512 x 480) ratios per second; 7) continuous electronic control of lamp intensity for UV applications; 8) fully integrated uncage and photobleaching functions; 9) capability for input of up to 8 analog channels; 10) ability to use macros for automating routines; and 10) dual dye technology for simultaneous experimentation. There is no other sourceknown by the NINDS researcher that can provide a system upgrade that is compatible with the existing Zeiss system and that has the aforementioned, required features. This is not a request for competitive quotation. However, if any interested party believes it can provide an equivalent upgrade, it may submit a statement of capabilities. The statement of capabilities and any other information furnished must be in writing and must contain material in sufficient detail to allow the NCI researcher to determine if the party can meet the above requirement. Capability statements must be received in the contracting office by 2:00 p.m. (Local Washington, D.C. time) on March 23, 2000 If you have any questions, please contact Debbie Moore, Purchasing Agent on (301) 402-4509. A determination by the government not to compete this requirement based upon responses to this notice is solely within the discretion of the government. Information received will be considered solely for the purpose of determining whether to conducta competitive procurement. Please reference NCI-RFQ-00072-NV on all correspondence and documentation. Posted 03/06/00 (W-SN431305). (0066)

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