National Cancer Institute, Research Contracts Branch, PSAS, 6120 Executive Blvd, EPS/Room 638, Bethesda, MD 20892-7227

66 -- INSTRUMENTS & LABORATORY EQUIPMENT( TOFSPEC 2E LASER DESORPTION FLIGHT MASS SPECTROMETER& ACCESSORIES) SOL RFQ-NCI-00075 DUE 032700 POC Cynthia Brown, Purchasing Agent, Todd Cole, Contacting Officer 301-402-4509 The National Institutes of Health, National Cancer Institute (NCI), Laboratory of Cell Biology, plans to procure a Tofspec-2E Laser Desorption Flight Mass Spectrometer on a sole source basis from Micromass, Inc., 100 Cummings Center, Suite 407N, Beverly, MA 01915. The Laboratory of Cell Biology is involved in the characterization of naturally processed peptides bound to MHC molecules and the identification of novel epitopes useful for immunotherapy. Peptides are highly pure and the analysis requires HPLC in combination with mass spectrometry. The NCI requires the use of a mass spectrometer to perform automated MALDI (Matrix Assisted Laser Desorption Ionization) experiments. Identification of post-translational modification in p53 and other proteins, plus the use of 2D electrophoresis and mass spectrometry for studying differential T cell signaling, will require the use of highly sensitive, state of the art equipment for approaches that are being developed at NCI. The Tofspec-2E will provide the NCI with a total MALDI ( Matrix Assisted laser Desorption Ionization ) mass spectrometry solution to protein identification. The required system shall be commercially available and have standard features such as: time-lag focusing, post-source decay, an optional collisional cell, sample viewing camera and monitor, positive and negative facilities, a resolving power of 10,000 FWHM, and the capability of data dependant instrument control for automated acquisition and data base searching. The system shall also have a unique on-axis single stage gridded reflectron to accommodate limited laboratory space. In addition, the system shall have a differentially pumped vacuum system (turbomolecular pumps) with a minimum of 480 total L/sec capacity to ensure adequate strength and sufficient vacuum for Time-of-Flight. Also, due to the limited space provided, the system shall have access to instrumentation for maintenance and service via a front panel, as opposed to the lengthy side panel. The software must have the ability to reliably select the 12C isotopes of peptides and deisotope and average all charge states to create an accurate mass to search. The instrument also offers a path for upgrading and adding a second laser. Micromass Inc. is the only source known to the NCI researcher that can provide such a system with all the above capabilities and features. This is not a request for competitive solicitation. However, if any interested party believes it can meet the above requirement, they may submit a statement of capabilities. All information furnished shall be in writing and must contain information in sufficient detail to allow the NCI researcher to determine if the party can meet the above specifications. Capability statements msut be received in the Contracting Office by 1:oo PM (local Washington, D.C. time) on March 27, 2000. If you have any questions, please contact Cynthia Brown, Purchsing Agent on (301) 402-4509. A determination by the Government ot to compete this proposed contract based upon responses to this notice issolely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. Please reference RFQ-NCI-00075. Posted 03/09/00 (W-SN432843). (0069)

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