National Institutes of Health, Office of Procurement Management, 6011 Executive Blvd., Rockville, Maryland 20892-7260

66 -- INSTRUMENTS & LABORATORY EQUIPMENT SOL 263-00-B-(GQ)-0018 DUE 040400 POC Dorothy Nickens, (301) 496-0301, Fax (301) 402-3407 Under authority of U.S.C. 253(c) (1), FAR 6.302-1 the National Institutes of Health (NIH), Bethesda, Maryland intends to negotiate on a sole source basis, a contract with Carl Zeiss, Inc, One Zeiss Drive, Thornwood, NY 10594. The National Institutes of Health, National Institute of Aging (NIA) in Baltimore, Maryland has a requirement for a real time digital fluorescence analyzer system. This system shall include, but not be limited to, the following performance characteristics, features, and pertain to the following components: Shall perform analysis of fluorescent probes in living cells and preparations. Shall be capable of visualizing ions in real time. Ions shall be observable individually or simultaneously, only the types of probes in use shall limit combinations. Shall include image intensified CCD (ICCD) and high resolution digital cameras. Shall include motorized inverted microscope and confocal module. Shall provide for simultaneous fluorescence and brightfield image acquisition. Shall include the capability for three ratio images per second in dual excitation configuration with a standard wavelength selector or better. Shall provide for ten ratios per second in static ratio imaging mode or better. Shall include upper and lower gray-value threshold functions. Shall provide for online background correction, ratio display, and shading correction functions or better. Shall provide for static ratio imaging and image averaging. Full frame ratio imaging shall be supported. Shall include image integration for very low light experimentation. Shall include provisions for data calibration. Shall include automated and interactive image storage. Shall include support for up to 99 regions of interest or better. Shall include experimental log and timer. Shall support stored image sequence analysis. Shall include software to integrate data and images. Shall include Z focus control. Shall be capable of performing ion studies in confocal mode in cells or tissues. The system shall be capable of providing three-dimensional image stacks of multiple probes. This shall allow for investigation of the structure and co-localization of antibodies. Shall support non-laser confocal fluorescence. Shall support ultraviolet and visible excitation wavelengths. Shall include PC based software for controlling the microscope hardware and digital imaging system. Shall include a Personal Computer with the following features, or better: Windows 98 software, 450MHz Pentium III processor, 128 megabytes of RAM, Two 17 inch monitors, Read/Write CD-ROM drive, Zip drive, LAN capable. The system shall be complete and perform the functions for which for which it was intended. All necessary interconnection cabling, documentation, and support after installation shall be provided. The system shall be compatible with existing Carl Zeiss Inc. equipment that has been previously acquired. The intended procurement will be classified under the Standard Industrial Classification Code (SIC) 3826, Laboratory Analytical Instruments with a size standard of 500 employees. Any response must be received in writing within fifteen (15) business days from the published date of this notice. Information furnished should include enough material to allow the Agency to perform a proper evaluation. Concerns that respond to this synopsis shall furnish concise responses directed specifically to the requirement mentioned above and are invited to submit capability statements in an original plus two (2) copies no later than fifteen (15) business days from the date of this announcement. Source demonstrating the capability to accomplish the above shall supply pertinent information in sufficient detail to demonstrate their ability to perform. Written response shall include complete description of the services to be provided, containing sufficient technical information to permit agency analysis to establish a bona fide capability to meet the stated requirements as listed above. This is not a request for proposal (RFP). Capability Statement: (a) If mailing your response through the U.S.Postal Service, use the following address: National Institutes of Health, Office of Procurement Management, 6011 Executive Blvd., Room 529M, MSC 7663, Attn.: Dorothy Nickens, Bethesda, Maryland 20892-7663; (b) If hand delivering or using a courier services (e.g. Federal Express, UPS, etc.,) use the following City, State and Zip code: Rockville, Md. 20852. Posted 03/14/00 (W-SN434162). (0074)

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