National Cancer Institute, Research Contracts Branch, PSAS, 6120 Executive Blvd, EPS/Room 638, Bethesda, MD 20892-7227

66 -- PCR PRMER PAIRS SOL NCI-RFQ-00112-NV DUE 052600 POC Debbie Moore, Purchasing Agent 301-402-4509, Todd Cole, Contracting Officer 301-402-4509 The National Cancer Institute (NCI), Division of Basic Sciences plans on procuring a set of PCR primer pairs, specifically Yeast Gene Pairs, from Research Genetics, 2130 Memorial Parkway, SW, Huntsville, AL 35801. The PCR primer pairs (Yeast GenePairs) are necessary in order to allow individual amplification of each of the annotated genes in the yeast (Saccharomyces cerevisiae) genome. The entire set of 6279 Yeast GenePairs will be referred to hereafter as the "Yeast Genomic GenePair Set". The Yeast Genomic GenePair Set is required to prepare a complete set of 6279 PCR products, which will be used to prepare high-density DNA microarrays containing every gene in the yeast genome for research on the study of gene regulation, gene function and chromosome structure. Although there are two types of microarrays currently in use for the analysis of yeast gene expression, the noted research requires in-house manufacturing using PCR products spotted onto glass slides with the existing PCR equipment for spottingand analyzing these arrays. The purchase of the Yeast Genomic GenePair Set will enable production of at least 200 PCR-product microarrays per year for several years. The following features are unique to this technology: 1) The 6279 primer pairs of the Yeast Genomic GenePair Set have been specifically designed to amplify a single yeast gene, and each primer pair is designed to contain a common priming sequence to allow subsequent re-amplification at the Yeast Genomic GenePair Set Control over content; 2) individual primers do not have to be designed since all necessary primers are together and have been concentration adjusted and tested for replication; 3) functionality has been tested and the primers have been used to amplify an entire genomic set of ORFs; 4) content can be controlled and modified to meet experimental needs, unlike commercially available microarrays; and 5) the Yeast Genomic GenePair Set can be used to identify protein binding sites at other places, including protein binding sites near the 5-prime ends and in the middle of genes, without bias to specific sites. The Yeast Genomic GenePair Set from Research Genetics is the only product known to the NCI Researcher that can meet all the above features necessary for the current research. This is not a request for competitive quotation. However, if any interested party believes it can meet the above requirement, it may submit a statement of capabilities. The statement of capabilities and any other information furnished must be in writing and must contain material in sufficient detail to allow NCI to determine if the party is providing a similar conference with similar subject matter, available expert participants, schedule, and size. An original and two copies of the capability statements must be received in the contracting office by 2:00 p.m. EST, on May 26, 2000. If you have any questions, please contact Debbie Moore, Purchasing Agent on (301) 402-4509. A determination by the Government not to compete this proposed requirement based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement Posted 05/09/00 (W-SN453134). (0130)

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