National Cancer Institute, Research Contracts Branch, ESS, Executive Plaza South, Rm. 620, 6120 Executive Blvd., MSC 7224, Bethesda, MD 20892-7224

B -- LABORATORY SUPPORT FOR PROCESSING AND STORAGE OF BIOLOGICAL SPECIMENS FROM PERSONS AT HIGH RISK FROM CANCER SOL N02-CP-11001-66 POC Shawnee Shaw, Contracting Officer Representative, (301) 435-3778 E-MAIL: Shawnee J. Shaw, SS552u@nih.gov. This is a SOURCES SOUGHT synopsis. The Office of the Director (OD), Division of Cancer Epidemiology and Genetics (DCEG), National Cancer Institute is seeking SMALL BUSINESS Sources with Standard Industrial Code (SIC) 8731 with a size standard of 500 employees to provide services in accordance with Contractor-developed, Government-approved protocols for: a) separation and viable cryopreservation of blood mononuclear lymphocytes; b) separation, aliquotting and storage of serum, plasma and/or urine; c) cryopreservation of bone marrow samples; d) storage of tumor extracts; e) cryopreservation of whole tumor tissue; f) cryopreservation of intact red blood cells; g) viable cryopreservation of previously established lymphoblastoid cell lines; h) storage of DNA and other biological materials as specified by the Project Officer (e.g., pathology slides and tissue block); i) extraction of DNA from biologic materials; j) specimen processing as required by NCI to preserve special biologic materials; k) logging in, labeling and tracking of each vial of each sample employing an NCI developed computerized specimen tracking system, including all laboratory safeguards to insure the fidelity and purity of each sample; and l) maintenance of the previously-established repository currently containing more than 2.0 million biological specimens and allowance for an estimated increase of up to 100% of freezer storage space. The current repository contains 72 mechanical 70oC freezers, 25 liquid nitrogen freezers, 2 walk-in 20oC freezers, 1 walk-in +4oC refrigerator, 1 slide cabinet and 1 storage unit for flammable materials; m) under the direction of the Project Officer conduct laboratory methods studies that establish optimal conditions for collection, processing, shipping and storage of biologic materials. Processing services shall be available routinely between the hours of 9:00 a.m. and 2:00 p.m., Monday through Friday, and at any other time (including nights, weekends and holidays) by special arrangement, usually with advance notice. A laboratory staff member shall be available during non-business hours for emergency specimen processing (as might occur when a patient dies). A biohazard area adequate for processing specimens with Acquired Immunodeficiency Syndrome (AIDS) shall be available for the processing of all biologic samples. The Contractor shall be prepared to process the following quantities of materials: Typical Total Max Daily Vials Monthly Volume Material /person /person (Individual Samples) WBC Cryopreservation 1,500 cc (60) 5 15,000 cc (250) Plasma/serum aliquotting 2,500 cc (60) 6-12 25,000 cc (250) RBC cryopreservation 250 cc (25) 5 750 cc (75) Pelleted lymphocytes 150 cc (15) 4 600 cc (60) Buffy coat preps 200 cc (20) 2 300 cc (30) Receipt frozen vials 10,000 vials any 120,000 vials Thawing/ aliquotting N/A any 500 vials Dispersal specimens N/A any 4,000 vials DNA purification N/A any 400 vials The Government envisions a staff composed of the following: 15 FTEs: 1) Principal Investigator 0.5 FTE; 2) Professional Staff 5.0 FTE; 3) Lab Technicians 6 FTE; 4) Data entry/admin 3 FTE; and 5) Driver 0.5 FTE. The contractor must be within 1 hour driving time to 6120 Executive Blvd., Rockville, MD. Capability statements from SMALL BUSINESSES ONLY (Original and 5 copies) shall include evidence of personnel and facilities capable of supporting this work. Submissions should be received at National Cancer Institute, Research Contracts Branch, 6120 Executive Blvd., EPS Room 620, MSC 7224, Attn: Shawnee J. Shaw, Rockville, MD 20892-7224 no later than 2:00 p.m. local time fifteen (15) days from date of publication of this announcement. This synopsis is for information and planning purposes only. It does not constitute a solicitation for bids or proposals, and is not to be construed as a commitment by the Government. The Government will not pay for any effort expended in responding to this synopsis. See Note 25. No collect calls will be accepted. Posted 05/11/00 (W-SN453780). (0132)

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