National Institute of Diabetes, Digestive & Kidney Diseases (NIDDK), Acquisition Management Branch, Natcher Building, 45 Center Dr., MSC 6600, Room 6AN32, Bethesda, Maryland 20892-6600

99 -- DEVELOPMENTAL BIOLOGIST/MOLECULAR GENETICIST SOL NIH-NIDDK-00-555 DUE 061600 POC Patricia Haun, Purchasing Agent, 301-594-8855 The National Institutes of Health (NIH), National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) has a requirement to purchase the services of a Developmental Biologist/Molecular Geneticist to culture, maintain and study Mutant Dictyostelium Cell Lines. The specific requirements are as follows: 1) Culturing of Dictyostelium cell lines: The candidate must have experience with media preparation and sterile techniques involved in the culture and transformation of Dictyostelium cells. Special expertise must include a knowledge of Dictyostelium development and genetics and of analyzing developmental phenotypes. 2) Cell transformation with genetic materials and assessment of gene transfer efficiencies: The candidate must be familiar with and able to perform gene transfer experiments of Dictysotelium. Expertise with preparation of transforming vectors and of knock-out transformation and ectopic gene expression Dictyostelium is essential. 3) Analysis of signal transduction: The candidate must have experience in receptor-mediated mechanisms of signal transduction. A complex understanding of receptor, G protein and protein kinase biochemistry is required. Expertise must involve receptor-ligand interactions, protein tyrosine kinase activity detection, and construction and expression of mutated genes and their analysis of their affect on receptor signaling. 4) Reagent storage and records: The candidate must be able to order and maintain the reagents necessary for the above requirements. The candidate must also be able to maintain the frozen laboratory cell stocks. The candidate must also be aware of precautions used in the handling of radioactive samples. A copy of the current Curriculum Vitae shall be included in the Request for Quote (RFQ) package. The anticipated period of performance will be a Base Year with one (1) option year period. All responsible small business sources and 8(a) sources may submit a quotation, which if timely received, shall be within the Simplified Acquisition Threshold for purchases up to $100,000. It is anticipated that RFQ NIH-NIDDK-00-555 will be available fifteen (15) days after the publication date of this synopsis, receipt of quotations will be due ten (10) days after the release of the RFQ. The anticipated award date is June 16, 2000. Requests for copies of the RFQ must be in writing and may be FAXED to 301-480-4226. Quotations must be in writing to the National Institute of Diabetes and Digestive and Kidney Disease: 6705 Rockledge Drive, Rockledge One, Suite 8048, Bethesda, MD 20817 Attention: Patricia Haun. FAX quotations must contain offerors signed by an authorized company representative. Posted 05/18/00 (W-SN456373). (0139)

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