National Institutes of Health, NIAID, Rocky Mountain Laboratories, 903 South 4th Street, Hamilton, MT 59840

68 -- OLIGONUCLEOTIDES SOL RML-RFQ-010 DUE 063000 POC Lynda Kieres, Purchasing Agent (406) 363-9210 / Rebecca Guenthner, Contracting Officer (301) 402-2284 E-MAIL: Lkieres@niaid.nih.gov, LKieres@niaid.nih.gov. This notice is a combined synopsis/solicitation for commercial items prepared in accordance with the format in Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation, proposals are being requested and a written solicitation will not be issued. This solicitation is being issued as a request for quotations (RML-RFQ-010). The solicitation documents and incorporated provisions and clauses are those in effect through Federal Acquisition Circular 97-17 (through 04/25/2000). This acquisition will be processed under Simplified Acquisition Procedures (SAP) and is not a Small Business Set-Aside. The associated Standards Industrial Classification Code (SIC) for this procurement is 2835 and the size standard is 500 employees. SCHEDULE: The Rocky Mountain Laboratories, Laboratory of Human Bacterial Pathogenesis (LHBP) proposes to procure: a complete oligonucleotide primer set specific for every open reading frame (ORF) of the Staphylococcus aureus genome from Sigma Genosys, 1442 Lake Front Circle, The Woodlands, TX, 77380. This primer set will consist of 6,000 primers for 3,000 ORFs. The primers shall be produced with the following features: (1) they shall be synthesized and aliquoted into 96 well format at 20 mM concentrations in water; (2) the forward and reverse primers for any gene shall be placed in different plates but in corresponding wells on those plates; (3) the first 2- 4 plates of primers shall be shipped within 3-5 days and 2-4 plates every day after that until the order is completed. 3,000 ORFs have been identified requiring 6,000 primers of 27 nucleotides in length. These primers will be used to amplify every gene of the S. aureus genome. Once amplified, these gene segments will be used to construct a DNA Microarray. Microarray analysis will be used to identify S. aureus genes that enable survival of the bacteria in the human host and contribute to pathogenesis in general. This will indicate genes which represent suitable targets for alternative therapies to staphylococcal infections. FOB Point shall be Destination, Hamilton, MT. Inside delivery shall be no more than 30 days after receipt of order (ARO). Delivery location is Rocky Mountain Laboratories, 903 S. 4th Street, Hamilton, MT. Sigma Genosys is the only company capable of performing the requirements in these volumes within the time frame required. The following FAR provisions and clauses apply to this acquisition: 52.212-1 Instructions to Offerors Commercial; Offerors must include with their offer a completed copy of the provisions at FAR 52.212-3 Offerors Representations and Certifications Commercial Items; FAR 52-212-5 Contract Terms and Conditions Required to Implement Statues or Executive Orders Commercial Items applies to this acquisition. The clauses of subpart (b), which apply, are 6 and 10. Copies of the above-referenced clauses are available upon request, either by telephone or fax. This is not a solicitation for competitive proposals. However, if any other interested party believes that it can meet the requirements it may submit a statement of capabilities to the POC indicated above (Fax -- 406-363-9288). Capability statements and related materials must be received in this office no later than 4:30 PM (MDST) on June 30, 2000. Posted 06/16/00 (W-SN465752). (0168)

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