Acquisitions Management Operations Branch, NIAID, 6700-B Rockledge Drive, Room 1130, Bethesda, MD 20892-7605

66 -- BIACORE 3000-OPTICAL BIOSENSOR SOL NOI-0019 DUE 081700 POC Sophia Janus, Purchasing Agent (301) 594-5223/John Foley, Contracting Office (301) 496-3878 This is a combined synopsis/intent to procure commercial items prepared in accordance with the format in FAR Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation, quotes are being requested, and a written solicitation will not be issued. This procurement is being issued as a notice of intent. Submit offers to NOI # 0019. The solicitation documents and incorporated provisions and clauses are those in effect through Federal Acquisition Circular 18. This acquisition is a 100% Small Business Set-Aside. The associated Standards Industrial Classification Code (SIC) for this procurement is 3826. SCHEDULE: The National Institute of Allergy and Infectious Diseases intends to procure a sole source simplified acquisition from Biacore, Inc. The equipment required is a Biacore 3000 instrument, product number BR-1100-43. The system includes the following: Operation Kit, various software, Sensor Chips, Reagents, CPU, monitor and printer, two-day training course and follow-up consultation. Biacore, Inc. is the sole distributor of Biacore instruments in the United States. A principle activity of the laboratory involves identifying host factors involved in AIDS pathogenesis. In this regard we need to acquire the capacity to define and characterize biomolecular interactions that occur between viral proteins and cell associated factors, including but not limited to cell surface receptors. The Biacore 3000 is designed to measure protein-protein interactions in real time. The Biacore technology has emerged as the state of the art methodology for measuring these interactions. The technology utilizes changes in surface plasmon resonace when biomolecules bind to, and dissociate from one another. This provides the distinct advantage of being able to measure both rates of association and dissociation between proteins. Additionally, biacore instruments have been improved recently such that "ligand fishing" experiments can now be carried out, thus opening up the possibility of identifying previously unknown interactions between viral proteins and host factors. The following characteristics make the BIACORE 3000 unique: 1. Sensitive SPR optical detection cabable of making reliable direct, real-time, label free measurements on as little as a few picograms of bound material, and detecting direct measurements of molecules as small as 200 Daltons. 2. Patented multichannel (up to 4 flow-cells) micro-fluidics integrated with an automated sample delivery system. Microrecovery of bound samples are possible for Mass Spectrometry applications. Patented, reusable sensor chip surfaces available in a variety of surface chemistries. 3. Assay temperature range of 4 to 40 degress C for thermodynamic analysis. 4. Evaluation software capable of global and statistical analysis of data for the following applications: specificity, kinetic, steady-state, and concentration. 5. BIACORE 3000 system specifications (see attached). 6. capable of micro recovery (less than 10ul) from the sensor surface. 7. Drift is less than 0.3 ru RMS and noise less than 0.1 ru. 8. able to reuse 50-100 times the same sensor chip for repeated sample analysis. 9. Dynamic range concentration (test times 5-10 min) 10-3 10-11 M detection limit approx. 1 pM. Delivery location is National Institutes of Health, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892 and shall be inside delivery. FAR provisions and clauses that apply to this acquisition: 52.212.-1 Instructions to Offerors Commercial Items; FAR 52.212.-3 Offeror Representations and Certifications- Commercial items; FAR 52.212.-4 Contract Terms and Conditions Commercial items; FAR 52.212-5 Contract Terms and Conditions Required to Implement Statutes or Executive Orders- Commercial Items. FAR 52.225.-3 Buy American Act. North American Free Trade Agreement Israeli Trade Act Balance of Payments Program. Offerors must include with their offer a completed copy of the provisions at FAR 52.212-3 Offerors Representations and Certifications Commercial items. The Government intends to make a Best Value Buy to the responsible Offeror whose offer is the most advantageous to the Government. See Numbered Note1. Evaluation will include 1.) The technical capability of the item offered to meet the agency need and 2.) Price. Warranty information to include period. Coverage shall be one year from the date of installation. Offeror must include with their offer certification that all hardware and software offered are Y2K compliant. Offers must be submitted not later than 3:00 PM, Eastern Daylight Time (EDT,) August 17, 2000, to Ms. Sophia S. Janus. Copies of the above referenced clauses are available upon request by telephone from Ms. Sophia S. Janus at (301) 496-2814. For delivery through the Postal Service the address is Room 1130, NIH/NIAID/AMOB, 6700B Rockledge Dr., MSC 7605, Bethesda, MD 20892-7605. If hand delivered or delivery service the address is Room 1130, NIH/NIAID/AMOB, 6700B Rockledge Dr., MSC 7605, Bethesda, MD 20817-7605. Electronic Submission will not be accepted. Requests for information concerning this requirement are to be addressed to Ms. Sophia S. Janus at (301) 496-2814. Collect calls will not be accepted. All responsible sources may submit an offer that will be considered by this Agency. Posted 08/03/00 (W-SN481727). (0216)

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