National Institute of Standards & Technology, Acquisition & Assistance Div.,100 Bureau Drive Stop 3572, Bldg. 301, Rm B117, Gaithersburg, MD 20899-3572

A -- ANALYTICAL CHEMISTRY METHOD DEVELOPMENT AND MEASUREMENTS SOL 43SBNB067090 DUE 082300 POC Sharon Blue, Contract Specialist e-mail sharon.blue@nist.gov, (301) 975-8831, e-mail sandra.febach@nist.gov, Sandra Febach, Contracting Officer (301) 975-6326. WEB: NIST Contracts Homepage, http://www.nist.gov/admin/od/contract/contract.htm. E-MAIL: NIST Contracts Office, Contract@nist.gov. The National Institute of Standards and Technology (NIST) intends to procure, on a sole source basis, the services of Susan Tai, of 822 Bald Cypress Drive, Rockville, MD 20850 under the authority of FAR 13.106-1(b) Only one source reasonably available. This procurement is for complete development and validation of LC/MS method for total serum thyroxine and serum cortisol. The contractor will complete method development for the determination of total serum thyroxine and cortisol using LC/MS and will rigorously evaluate the accuracy and precision of the method to assure that it meets the high standards of NIST. The method will be applied to selected materials for measurement. The determination of free thyroxine, representing about 0.1% of the total thyroxine must be investigated to determine if a method can be established to isolate free thyroxine from protein-bound thyroxine. The method will be applied to the determination of cortisol in selected materials. The method development will include extractionof thyroxine and cortisol from serum in high yield and without decomposition, further processing of the extract to remove potential interferences, liquid chromatographic separation, and electrospray ionization mass spectrometry for measurement. The contractor must have demonstrated skill in developing high accuracy methods for trace level analytes in biological matrices and must have expertise in the chemistry of thyroxine and cortisol, in organic isotope dilution, in modern liquid chromatography, and in electrospray ionization mass spectrometry. The methods must be tested by repeated measurements of thyroxine in pools of human serum with thyroxine levels that are representative of the range encountered in normal and thyroid-impaired human populations, and of cortisol in pools of human serum with cortisol levels that are representative of the range encountered in normal human populations as well as those with elevated or depressed endocrine function. Alternate measurement strategies must be devised to test for bias in the measurements. Comparison with field method results will be included to assure that there is commutability between the methods. The methods must be applied to the determination of thyroxine and cortisol in SRM materials and any other serum materials that the COTR deems necessary. This is a follow on project where Susan Tai worked under a CRADA that has expired, with the College of American Pathologists performing the same tasks. Susan Tai has knowledge and experience in all of the areas mentioned. She understands the stringent accuracy and precision requirements that are expected of NIST methods and measurements. Award to another source would result in substantial duplication of cost that the Government would not recover through competition and would create unacceptable delays in fulfilling NIST's mission requirements. It is anticipated that a Firm Fixed Price Purchase Order will be issued by September 11, 2000. The Period of Performance is for one year from the date of award. Interested persons may identify their interest and capability to respond to the requirement. This notice of intent is not a request for competition. However, the Government will consider all responses received within 15 days after the date of publication of this synopsis. Written responses to this synopsis shall contain sufficient documentation to establish a bonafide capability to fulfill the requirement. A solicitation is not available. Posted 08/09/00 (W-SN483904). (0222)

Loren Data Corp. http://www.ld.com (SYN# 0006 20000811\A-0006.SOL)

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