DHHS, PHS, CDC, NIOSH, ALOSH, 1095 Willowdale Rd., Morgantown, WV 26505-2888

Q -- GENERATION OF A KNOCKOUT MOUSE MODEL SOL RQ2000-034 DUE 082500 POC Brenda Goodwin, 304/285-5882; Contracting Officer, Rex S. Wolfe, Jr., 304/285-5883 NOTICE OF INTENT TO SOLE SOURCE The National Institute for Occupational Safety and Health (NIOSH), Toxicology and Molecular Biology Branch, HELD in Morgantown, WV is currently studying the function of a novel gene (T1G1) that is inducible by dioxin and may contribute to the etiology of dioxin toxicity. Current analysis of the gene suggests that T1G1 is regulated during tumor formation in lymphoid tissues and memory processes in the brain. Generating a knock out mouse model in which the gene of T1G1 is altered or deleted would permit genetic study of the physiological function and the role in dioxin toxicity of T1G1. This work requires a company specialized in gene targeting with state of the art equipment and technology. The only resource known to have the capabilities to generate a knockout mouse is inGenious Targeting Laboratory, Inc., Stony Brook, NY. This corporation will perform cell work, electroporation, and screening of clones until identification and confirmation of positive ES clones is accomplished. This corporation guarantees a successful germline transmission once a positive ES clone is identified and provides F1 heterozygous mice instead of the chimera founder mice. Once the gene targeting services for this project are performed and the F1 heterozygot is obtained, the gene targeting laboratory has no ownership to the animals and no royalties are ever collected. If germline transmission is not achieved, the company does not require payment for performance of unsuccessful gene targeting services. The facilities house animals in maximum isolation and every item that enters the isolation rooms is autoclaved. Mice are maintained under specific pathogen-free conditions and serology tests are performed every 6 weeks where health reports are provided. There is no other known source available, however, if there are vendors having the capabilities necessary to meet the stated requirements, they are invited to submit appropriate documentation, literature, brochures and references to Brenda L. Goodwin, Reference: RQ2000-034, DHHS, PHS, CDC, NIOSH, ALOSH, 1095 Willowdale Road, Morgantown, WV 26505. The Government intends to negotiate on a sole-source basis with inGenious Targeting Laboratory, Inc., Stony Brook, NY. Interested organizations must provide a description in sufficient detail to show that the services being offered demonstrate adequate qualifications/capabilities and a successful record of past performance. To be considered, responses shall be submitted within 14 days after publication of this notice. Information received will generally be used for the purpose of determining whether to conduct a competitive procurement. This notice is issued for informational purposes only. Posted 08/11/00 (W-SN484960). (0224)

Loren Data Corp. http://www.ld.com (SYN# 0061 20000815\Q-0013.SOL)

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