National Institute of Diabetes, Digestive & Kidney Diseases (NIDDK), Acquisition Management Branch, 6705 Rockledge Dr., MSC 7973, Room 8048, Bethesda, Maryland 20892-7973

66 -- AKTA BASIC 10 (OR EQUIVALENT) LIQUID CHROMATOGRAPHY SYSTEM SOL NIH-NIDDK-00-576 DUE 091500 POC Patricia Haun, Purchasing Agent, 301-594-8855 The National Institutes of Health (NIH) National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) has a requirement to purchase a AKTA Basic 10 (or equivalent) Liquid Chromatography System and accessories. This equipment is needed for the ability to purify biomolecules including peptides, nucleic acids, and proteins from complex starting materials derived from cell lysates, tissue homogenates, recombinant protein expression systems and other sources. The system is needed to perform separations based on a variety of sample characteristics, such as molecular weight, net surface charge, hydrophobicity, while retaining biological activity. The system must have the following specifications: 1) A P-903 (or equivalent) two-pump system that can provide a flow rate range of 0.001 to 10 ml/min and a pressure range from 0-25 MPa (3625psi); 2) The two-pump gradient system shall contain two switch valves to allow for accurate, automatic buffer preparation; 3) The two-pump gradient system, with dynamic mixing, provides shallow gradients for high-resolution separations of biomolecules. The two-pump and mixer configuration produces high accuracy and precision in gradient formation; 4) A M-925 (or equivalent) mixer. A single chamber mixer powered and controlled from the P-903 (or equivalent) pump. The mixer has interchangeable mixing chambers of 0.6 and 2 ml; 5) The system shall have a UV-900 (or equivalent) monitor that can simultaneously monitor three different wavelengths in a range of 190-700nm. The UV monitor bandwidth is 4nm with a wavelength accuracy of plus or minus 2nm. The UV monitor includes a 10mm flow cell; 6) The system shall include a seven port INV-907 (or equivalent) injection valve with load, inject and wash positions; 7) The system shall include an eight port PV-908 (or equivalent) motor fractionation valve for diversion of the sample to the fraction collector or waste; 8) The system shall have a Frac-901 (or equivalent) fraction collector to allow fixed volume fractionation or automatic peak fractionation. Peak fractionation can be based on peak detection using slope or level sensing. The Frac-901 (or equivalent) collects up to 175 fractions in 12mm diameter tubes or up to 95 fractions 10-18mm diameter tubes; 9) The system computer and software shall monitor two pumps, two motorized valves, a fraction collector, a dynamic gradient mixer, an UV monitor, an autosampler and a conductivity/pH monitor. All responsible sources may submit a quotation, which if timely received, shall be within the Simplified Acquisition Threshold for Purchases up to $100,000. Vendors capable of furnishing the government this equipment should request a copy of the Request for Quote from the above contact. It is anticipated that the Request for Quote (RFQ) NIH-DDK-00-576 will be available fifteen (15) days after the publication date of this synopsis. Receipt of quotations will be due ten days (10) after the release of the RFQ. The anticipated award date is September 19, 2000. Request for copies of the RFQ must be in writing and may be FAXED to 301-480-4226. Quotations must be submitted in writing to the National Institute of Diabetes and Digestive and Kidney Diseases 6705 Rockledge Drive, Building One, Room 8048, Bethesda, Maryland 20817-7973 Attention: Patricia Haun. FAX quotations must contain offerors names, address, phone number and fax number. FAX quotations will only be accepted if dated and signed by an authorized company representative. Posted 08/17/00 (W-SN487380). (0230)

Loren Data Corp. http://www.ld.com (SYN# 0277 20000821\66-0015.SOL)

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