USDA-ARS-National Animal Disease Center, 2300 Dayton Avenue, P.O. Box 70, Ames, IA 50010

66 -- 00 -- SMARTCYCLER SOL 00-87 DUE 083100 POC Mary Welch 515-663-7415 FAX 515-663-7482 The National Swine Research Center has a requirement TO purchase a Smartcycler and Smartcycler reaction tubes. This is a combined synopsis'solicitation for a commercial item, prepared in accordance with the format in FAR Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; quotes are being requested and a written solicitation will not be issued. This solicitation is being issued as a Request for Quotation (RFQ) 87-00. This solicitation is under the simplified Acquisition procedure (SAP) and is a Small Business- Small Purchase Set-aside. The Standard Industrial Classification (SIC) code is 3826 and the Business Size Standard is 500 employees. This will be a firm-fixed price contract. CLIN 001: Smartcycler starter system, (1 ea.) Smartcycler reaction tubes (2 ea) or equal THE FOLLOWING ARE THE MUST HAVE SPECIFICATIONS: 1.FLUORESCENT OPTICAL DETECTION WITH CAPABILITY TO MEASURE UP TO 4 SPECTRAL BANDS USING HIGH INTENSITY LED EMITTERS AND SILICON PHOTODETECTORS. 2. REACTION SITE THERMAL CONTROLS WITH THERMISTORS CALIBRATED TO + OR -- DEGREE C AND SOLID STATE HEATER AND FORCED-AIR COOLING. 3. THERMOCYCLER WITH 16 INDIVIDUALLY PROGRAMMABLE REACTION SITES EACH CAPABLE OF VARYING TIME OF REACTION, TEMPERATURE OF REACTION, AND RAMPING RATE OF HEATING AND COOLING WITH THE FOLLOWING PERFORMANCE PARAMETERS; HEATING RAMP RATE (MAX.) 10 DEGREES C/SEC FROM 50 DEGREES C TO 95 DEGREES C, COOLING RAMP RATE (MAX): 2.5 DEGREES C/SEC FROM 95 DEGREES C TO 50 DEGREES C, TEMPERATURE DURATION ACCURACY: 1.0 SEC FROM PROGRAMMED TIME, TEMPERATURE ACCURACY +/- FROM 60 DEGREES C TO 95 DEGREES C, MELT CURVE PROGRAMMABLE RAMP RATES; 0.1 DEGREES C/SEC TO 1.0 DEGREES C/SEC 4. MUST BE EXPANDABLE IN SIXTEEN-UNIT INCREMENTS UP TO A TOTAL OF 96 REACTIONS SITES. 5. SOFTWARE SHOULD BE CAPABLE OF QUANTITATIVE AND QUALITATIVE ANALYSIS; A. QUALITATIVE-MELT CURVE ANALYSIS. B. QUANTITATIVE-GROWTH CURVE AND SECOND DERIVATIVES. C. DATA EXPORTABLE TO MS EXCEL-COMPATIBLE SOFTWARE 6.WITH THE FOLLOWING ASSAY PERFORMACE: A. CYCLE TIME (BASED ON B-ACTIN ASSAY) <69 SECONDS/CYCLE, <42 MIN FOR 40 CYCLES B. NUMBER OF CYCLES TO DETECTION (B-ACTIN ASSAY); 150,000 INITIAL COPIES DETECTED IN <28 CYCLES, 150 INITIAL COPIES DETECTED IN <34 CYCLES, <150 INITIAL COPIES DETECTED IN 35-45 CYCLES. QUOTING ON FISHER P/N 11-400 AND 11-400-2 OR EQUAL, Manufacturer's data, descriptive material, or other information on the equipment to be furnished under this request for quotations. Per FAR 13.106-2, award will be made to ensure that the purchase is the most advantageous and of the best value to the Government; based on the following evaluation factors. The Government will select the factors considered to be significant to this purchase; 1. technical Capability of the items offered to meet the Government's requirement; 2. Price; 3. Past Performance; 4. Warranty Conditions; 5. Maintenance Availability; 6. Quality Reference of three (3) firms for which you have provided similar equipment are requested. These will be used in the Evaluation process. Delivery F.O.B. Destination Price. Equipment to be delivered to the following address; National Swine Research Center, 2150 Pammel Drive, Ames, Iowa 50011, The provisions at 52.212-4 Contract Terms and Conditions -- Commericial Items; and 52-212-5, Contract terms and Conditions Required to Implement Statutes or Executive Orders -- Commercial items apply to this acquisition. Offerors must include a completed copy of the provision at 52.212-3, offeror Representations and Certifications -- Commercial Items, with its offer. SEE Note 1. Posted 08/17/00 (W-SN487251). (0230)

Loren Data Corp. http://www.ld.com (SYN# 0279 20000821\66-0017.SOL)

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