USDA, ARS, MSA, Procurement/Assistance Office, P.O. Box 225, Stoneville, MS 38776-0225

66 -- SCAN ARRAY BIOCHIP SCANNING SYSTEM SOL 2000-4431-024 DUE 092400 POC Terry A. Krutz, 662-686-5361 The USDA, ARS, Mid South Area, Biological Control and Mass Rearing Research Unit, Stoneville, Mississippi has a requirement for a GSI Lumonics, ScanArray 5000XL Biochip scanning system. This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; quotations are being requested and a written solicitation will not be issued. Solicitation No. IFB 2000-4431-024 is being issued as an Invitation for Bid. This solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular 97-12. Offerors are to provide prices for the following: (1) 1ea -- ScanArray 5000XL Standard Biochip Scanning System (100 -- 240V) which includes these specifications -- Software controlled confocal scanning laser system for the analysis of fluorescently labeled microarray biochips. The scanning system must meet the following specifications/requirements and include all the equivalent parts listed by GSI Lumonics catalog number and description. 1ea ScanArray5000XL, ScanArray 5000XL standard Biochip Scanning System with ScanArray Acquisition software, network communication cable, instruction manual and power cords. 1ea QuantArray Microarray Analysis Software -PC, 1ea Shipping, Warranty: One-year parts and labor, Set-up, Installation and one day on-site training with follow up training. System Specifications: Confocal five-color scanning system with bi-directional linear translation slide positioning for the purpose of acquisition and analyzing gene expression data from microarrays. System is to be upgradeable with additional laser, alternative lasers and autoloader. Specifications: Sample size: Standard or metric slides, 1" x 3" (25 x 76 mm), Scanning Field: 22 mm x 73 mm, Pixel Resolution: Multi-resolution scanning, 5, 10, 20, 30 and 50 um, Excitation Wavelengths: Variable from 488 to 633nm, Emission Wavelengths: Variable from 500 to 700nm, Sensitivity: < 0.1 molecule fluor/ um2, Scan Speed, 20 scan lines / second < 5 minutes for full field scan 2 second slide ejection / retraction, Dynamic range: 16-bit dynamic range = 5 orders of magnitude, Image File Formats: TIFF, BMP, and Raw, Size: Scanner -approx. 30"L x 16"W and 14" H External Laser -approx. 29"L x 13"W and 12"H, Power: Scanner: 208 V, 60Hz External Laser 208V, /60Hz U.S. 20 amp. Functional Specifications: Fluorescent dyes compatibility: AlexaTM488, 532, 546, 568 and 594, Fluorescein, Rhodamine, 5-FAM, TAMARA, JOE, ROX, Texas Red. Sample type compatibility: Standard and metric glass microscope slides; membranes, plastics and reflective metals; wet and dry samples, with and without covers slips; commercially available biochips, including NEN MICROMAX System. Lasers: Configuration-five (5) lasers: 488nm and 514nm (Argon) external, and 543nm(GHeNe), 594nm(YHeNE), and 633nm(RHeNe) internal. Laser excitation range: 488nm to 633nm, Laser excitationrange available: 488nm (Argon), 514nm (Argon), 543nm(GHeNe), 594nm (YHeNE), 612nm(OrHeNe), 633nm (RheNe), System is to have choice of up to 11 emission channels from 500nm to 633nm compatible with selected lasers. Scanning resolution: 5 to 50 um, System is to be capable of scanning 22 mm x 73mm in < 5 minutes. System is to be capable of real time characterization of excitation and emission light to allow for instrument-to-instrument normalization and calibration over time and temperature. System is to be upgradeable with alternative internal and external lasers and autoloader. External/Internal Lasers: Computer controlled with automatic laser monitoring, calibration and adjustment, Laser sleep modes to extend laser life, Easy laser coupling and automatic alignment, Meet all laser safety/emissions requirements, Detection ability should include, but not be limited to the following dyes: FITC, FAM, Alexa 532, Tetramethyl Rhodamine, Alexa 546, Texas Red, ROX, ABI JOE, Alexa 594, Cy5, Cy5.5, BODIPY 630/650, TO-PRO, ABI TAMRA ABI ROX, Sensitivity is to be < 0.1-molecule fluor/um2. Barcode Reader: System is to have barcode reader that can define scanning parameters automatically. Labels are to be detectable on top and bottom of samples. System is to be capable of using alphanumeric characters to define and track gene information. Labels are to be resistant to microarray preparation, washing and hybridization. System Automation: Automatic four color imaging and experimental protocols allowing for 24 hr operation. Automatic channel range and balancing. Laser sleep mode. Multiple image summing and averaging feature. Autofocus. User defined background subtraction and dark current correction. High-speed TCP/IP network interface. Analysis Software Specifications: Fully automated of two, three and four-color microarray images using user-defined analysis protocols. Seamless integration into ScanArray acquisition software. One-Step scanning and quantitation with Automation Module. Auto-adjustable grid feature to fit most microarrays. Automatic and use-defined data extraction and export to Excel and ASCII formats. Scatter plot, pie, bar, histogram, threshold and statistical graphical visualization. Three quantitation methods (Histogram, Fixed Circle, Adaptive) and quantitative pixel display. Automated cross-talk correction for signal and background calculations. Image annotation assigns gene names and allows for text and object placement on images. Import of spotting parameters associates spot locations to gene name. Delivery of the equipment shall be made within 45 days after receipt of the contract order and will be delivered to USDA, ARS, MSA, Biological Control and Mass Rearing Research Unit, Stoneville, Mississippi 38776. Prices should reflects that they are FOB Destination. Price will include everything identified above. FAR 52.212-1, Instructions to Offerors -- Commercial, FAR 52.212-2, Evaluation- Commercial Items; FAR 52.212-3, Offeror Representations and Certifications -- Commercial Items; FAR 52.212-4, Contract Terms and Conditions -- Commercial Items; FAR 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders -- Commercial Items apply to this acquisition. These clauses maybe viewed at http://www.arnet.gov/far/ Quotations will be evaluated on price, past performance, and proof of experience with this kind of equipment. Offers should include a list of at least four references to include company name, point of contact, phone number and fax number. References should reflect similar type of work or equipment purchases. Additional Quotation Information -- Descriptive Literature must be submitted with the offer even if is has been submitted previously. This literature MUST address all aspects outlined in the specifications. If an area is not addressed in the literature the company must submit a written substantive statement describing such area. FAILURE TO PROVIDE THE DESCRIPTIVE LITERATURE SHALL RESULT IN REJECTION OF YOUR OFFER. Offers to include a commercial warranty for equipment. Offer must include a 30 calendar day acceptance period. Offerors are to be received not later than 3:30(CST), September 14, 2000 at the USDA, ARS, MSA, Contracting Office, 141 Experiment Station Road, P. O. Box 225, Stoneville, MS 38776-0225. Fax quotations are acceptable. FAX No. (662) 686-5373. The POC for this project is Terry A. Krutz (662) 686-5361. Posted 08/21/00 (W-SN488159). (0234)

Loren Data Corp. http://www.ld.com (SYN# 0290 20000823\66-0020.SOL)

| 66 - Instruments and Laboratory Equipment Index  |  Issue Index |