National Institute of Standards & Technology, Acquisition & Assistance Div.,100 Bureau Drive Stop 3572, Bldg. 301, Rm B117, Gaithersburg, MD 20899-3572

R -- PREPARATION OF BIOLOGICAL SAMPLES FOR USE WITH OPTICAL TWEEZERS SOL 52SBNB060169 DUE 091100 POC Marsha Rodgers, (301)975-6398, E-mail Marsha.Rodgers@nist.gov, fax (301)963-7732, Contracting Officer Sandra L. Febach WEB: NIST Contracts Homepage, http://www.nist.gov/admin/od/contract/contract.htm. E-MAIL: NIST Contracts Office, Contract@nist.gov. The National Institute of Standards and Technology (NIST) has a requirement for a contractor to provide services to prepare biological samples for manipulation and quantification using optical tweezers. The contractor shall perform the following tasks: 1) Be responsible for producing samples to be used in a laser trapping and dissection apparatus (optical tweezers and optical scissors, respectively), 2) Prepare the samples in a vessel with medium suitable for use in a laser trapping apparatus, and 3) Make measurements using the samples in the laser trapping and dissection apparatus. Deliverables shall include preparations of cells, liposomes and microspheres coated with biomolecules, as required for use in the laser trapping experiments, data on measurements of adhesion forces and rate constants between binding pairs of biomolecules and monthly progress reports which contain the specific protocol for sample preparation as well as specific details of the measurement procedures. Work shall be performed on-site at the NIST Gaithersburg, Maryland site. The contractor shall meet the following minimum experience requirements: 1) The contractor shall have a Masters or higher degree in biochemistry or a biochemistry related field; 2) The contractor shall have 3 or more years of experience in the covalent coupling of antibodies and antigens to microspheres; 3) The contractor shall have experience in the covalent coupling of fluorescent molecules to biomolecules and microspheres; 4) The contractor shall have experience in separation and handling of leukocytes from whole blood; 5) The contractor shall have experience in the preparation of micron-sized liposomes; 6) The contractor shall have experience in working with a laser trapping apparatus (optical tweezers); 7) The contractor shall have experience in measuring adhesion forces between binding pairs of biomolecules; and 8) The contractor shall have experience in measuring rate constants for adhesion between binding pairs of biomolecules. Evaluation criteria shall be Understanding the Technical Requirement and Experience of Key Personnel. F.O.B.Destination, Gaithersburg, Maryland. The Period of Performance shall be one year from date of award. There shall also be four additional one year option periods. All responsible sources may submit a proposal which shall be considered by the agency. Solicitations shall be available at: http://www.nist.gov/admin/od/contract/contract.htm on or about September 1, 2000. Written solicitation requests are required in writing and may be telefaxed to (301) 963-7732. Posted 08/19/00 (W-SN488041). (0232)

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