66 -- GENEAMP 5700 SEQUENCE DETECTION SYSTEM

Notice Date

September 14, 2000

Contracting Office

Directorate of Contracting, Uniformed Services University of the Health Sciences, Rm A1040c, 4301 Jones Bridge Road, Bethesda MD 20814-4799

ZIP Code

20814-4799

Solicitation Number

MDA905-00-Q-0092

Response Due

September 30, 2000

Point of Contact

David Denton, 301-295-3068, or fax 301-295-1716

E-Mail Address

CLICK HERE TO CONTACT THE CONTRACTING OFFICER (ddenton@USUHS.MIL)

Description

The Uniformed Services University of the Health Sciences (USUHS), Bethesda, MD requires a GeneAmp 5700 Sequence Detection System and all associated components from the manufacturer PE Biosystems. This item will be procured on a brand name o equal basis. The GeneAmp 5700 Sequence Detection System is a complete system for real-time quantitative PCR. The system includes a GeneAmp 9600 thermal cycler, precision optics, computer, and application software for reproducible quantitation of nucleic acid sequences. Results are available directly after PCR with no additional purification or analysis steps. Multiple Assay Chemistries Real-time quantitation can be performed on the GeneAmp 5700 Sequence Detection System using either the fluorogenic 5' nuclease assay or the double-stranded DNA binding dye SYBR Green I. Real-time assays using the SYBR Green I dye are ideally suited for rapid screening and target discovery as no specific probes are needed. The fluorescence of SYBR Green I Dye increases when bound to double-stranded DNA, offering a very simple way to monitor PCR product accumulation. The use of a fluorogenic probe in a TaqMan assay provides outstanding specificity by eliminating the detection of PCR artifacts. With this chemistry, a TaqMan probe complimentary to the target sequence is added to the PCR reaction mixture. If the target sequence is present during PCR, the TaqMan probe anneals and is cleaved by the 5' nuclease activity of AmpliTaq Gold DNA Polymerase. This results in an increase in reporter dye signal which is used to monitor specific PCR product accumulation. Installation Specifications: The beta-actin installation procedure guarantees that the GeneAmp 5700 Sequence Detection System has the ability to distinguish between 2,000 and 10,000 genome equivalents with a 99.7% confidence level for a subsequent sample run in a single well. It should be noted that two-fold resolution can be achieved by running samples in replicate wells. The number of replicate wells required is dependent upon the performance of the specific assay and the required confidence level. System Performance: Throughput: Quantitation of 96 samples per run. Data analysis is complete in less than 1 minute. Dynamic Range: At least five orders of magnitude in starting copy number. System Components GeneAmp 5700 Sequence Detector. Light source is a tungsten-halogen lamp. Fluorescence signal is detected via a CCD camera, with a detection range of 530-580 nm. Dell Computer. 64 MB RAM, 400 MHz Pentium II processor, 6.4 GB hard disk drive, 14X CD-ROM drive, 1.44 MB floppy disk drive, 15" color monitor, Windows NT 4.0 operating system. Sequence Detection System Software. The GeneAmp 5700 Sequence Detection System Software is used for system control, data collection and data analysis for real-time quantitative PCR. GeneAmp PCR System 9600. Industry standard 96-well thermal cycler. Its proven compressor-based technology provides the basis around which most PCR amplification protocols have been developed. This notice is a combined synopsis/solicitation for commercial items prepared in accordance with the format in subpart 12.6 the Federal Acquisition Regulation (FAR) part 12, Commercial Acquisition and FAR part 13.5 supplemented with additional information included in this notice. This announcement constitutes the solicitation; proposals are being requested and a written solicitation will not be issued. This notice is being used as request for proposal for: Clin 0001) GeneAmp 5700 Sequence Detector System: installation/onsite training, Dell Computer with Pentium Processor; sequence detector software; primer/probe design software, user's manual, and consumables. DELIVERABLES: The system shall be delivered and installed at USUHS, Bethesda, MD 20814-4799, and FOB: Destination within 90 days. Delivery shall be made within the hours of 8:00 a.m. to 4:00 p.m., Monday through Friday, excl. Federal holidays, and shall be scheduled with the COR named at the time of award. PACKING and SHIPPING: All items packed and shipped shall have the mailing label addressed as follows: USUHS (complete address) and the contract number (named at the time of award). INSTALLATION: The contractor shall be responsible for the movement of all equipment to installation site. The Contractor shall install the microscope, complete with the components. The Contractor shall also perform verification testing and provide technical support. Installation shall be completed within 30 days after delivery to USUHS and is to be coordinated with the COR (to be named at time of award). SET-UP and TRAINING: The Contractor shall be responsible for performing all start-up operations to ensure the system is fully functional for its intended purpose. The contractor will provide least three full days of operator training in the laboratory at USUHS within 15 days after completion of installation. DOCUMENTATION: Upon completion the contractor shall provide two (2) sets each of all equipment/software manuals for the GeneAmp 5700 sequence detection system. Final payment shall not be made until such documentation is received and accepted by the Contracting Officer. WARRANTEE: A standard commercial warranty shall be provided for the software and equipment. The Contractor shall extend to the Government the full coverage of any standard commercial warranty normally offered in a similar commercial sale, provided such warranty is available at no additional cost to the Government. Acceptance of the standard commercial warranty does not waive the Government's rights under the Inspection clause nor does it limit the Government's rights with regard to the other terms and conditions of this contract. The provision of FAR 52-212-1, Instruction to Offerors -- Commercial Items and 52.211-6, Brand Name or Equal, applies to this acquisition. All offerors are required to submit a completed copy of Addenda to FAR Clause 52.212-1 1) Item (b) Submission of Offers is amended to include the following: Submit your proposal, acknowledgment of amendments (if any), software licensing agreements (if any), descriptive literature for the proposed equipment and software, and the FAR 52.212-3 -- Offeror Representations and Certifications -- Commercial Items, prior to an award. Telegraphic or facsimile offers are not acceptable. Period for acceptance of offers is amended to include the following: The offeror agrees to hold the prices in its offer firm for 90 calendar days from the date specified for receipt of offers. The provision at 52-212-1, Instruction to Offerors -- Commercial Items, applies to this acquisition. The provision of FAR 52.212-2, Evaluation -- Commercial Items, applies to this acquisition. The following evaluation criteria are included in paragraph (a) of that provision. Addendum to FAR clause 52.212-2. Evaluation Factors for award: Any resulting order will be issued to the company offering the best value to the Government, price and other factors considered. Factors that will be considered are Technical capability and price. Technical capabilities are significantly more important than price. Failure to provide the information relative to each evaluation factor may render offer non-responsive. 1) TECHNICAL CAPABILITY: Technical capability will be based on product literature, technical features and/or warranty provisions. Each offeror is required to submit licensing (if applicable), descriptive literature, or other documentation, manufacturer or brand name and model of the item, and show how the offered product meets or exceeds the requirements as specified in the Specifications. 2) PRICE: Price includes all equipment, software, software licensing, training, warranty costs, discount terms, and transportation costs. Evaluation is based on best value including cost and ability to meet stated requirements above. Offerors shall submit descriptive literature and drawings detailing features, technical capabilities and warranty data. Technical acceptability will be determined solely on the content and merit of the information submitted response to this provision as it compares to the minimum characteristics provided above. Therefore, it is essential that offerors provide sufficient technical literature, documentation, etc., in order for the Government evaluation team to make an adequate technical assessment of the quote as meeting technical acceptability. The full text of these clauses may be accessed electronically at this address: http://www.arnet.gov/far/. Offerors shall include a complete copy of the provision at FAR 52.212-3, Offeror/Representation and Certifications-Commercial Items. The following FAR clauses cited in 52.212-5 are applicable to this acquisition for supplies: 52.203-6, Restrictions on Subcontractor Sales to the Government (JUL 1995); 52.222-21, Prohibition of Segregated Facilities (FEB 1999); 52.222-26, Equal Opportunity (APR 1984); 52.222-35, Affirmative Action for Disabled Veterans and Vietnam Era Veterans (APR 1998); 52.222-36, Affirmative Action for Workers with Disabilities (JUN 1998); 52.222-37, Employment Reports on Disabled Veterans and Veterans of the Vietnam Era (JAN 1999); 52.225-3, Buy American Act Supplies (JAN 1994); 52.225-13 Restrictions on certain Foreign Purchases (FEB 2000); 52.225-21, Buy American Act North American Free Trade Agreement Implementation Act Balance of Payments Program (JAN 1997); 52.232-33, Payment by Electronic Funds Transfer Central Contractor Registration (May 1999); 52.239-1, Privacy or Security Safeguards (AUG 1996). ALL INTERESTED PARTIES SHALL SUBMIT OFFERS WITH THE FOLLOWING INFORMATION: Federal Tax Identification (TIN); Dun & Bradstreet Number (DUNS); remit to address if different; and business size. As of 31 May 1999 all Contractors must be registered in the Central Contractor Registration (CCR) Database as a condition for contract award. Contractors may register in the CCR through the World Wide Web at http://www/acq/osd.mil/ec or call the DOD Electronic Information Center at 1-800-334-3414.

Record

Loren Data Corp. 20000918/66SOL009.HTM (W-258 SN497012)