A -- DNA ISOLATION AND MOLECULAR ANALYSIS

Notice Date

December 6, 2000

Contracting Office

National Institutes of Environmental Health Sciences, Research Contracts Branch, DERT, P.O. Box 12874, Building 4401, Research Commons, 79 T.W. Alexander Drive, Research Triangle Park, NC 27709

ZIP Code

27709

Solicitation Number

NIH-ES-01-02

Point of Contact

Contact Velvet M. Torain, Contract Specialist, 919/541-0400/Fax No. 919/541-2712

E-Mail Address

None (None)

Description

This project will require both analysis of large numbers of samples (up to 5000) for a single genotype and small numbers of samples (up to 500) for multiple gentotypes (an average of 10). Need for an inventory of methods for up to several hundred polymorphisms is foreseen. A successful offeror will demonstrate the ability to work closely with the Project Officer and Government Task Leaders to develop and validate assays for newly identified polymorphisms. A successful offeror will also demonstrate that the proposed genotyping methods (s) can be rapidly and easily adapted to new polymorphisms and are rapidly scaleable to deal with increasing numbers of new polymorphisms. The offeror should demonstrate capability to identify several types of polymorphisms, including single nucleotide polymorphisms (SNPs), repeat length polymorphisms including nucleotide repeats, insertions, and deletions. It will be required to provide a detailed standard operating procedure to protect the safety and health of individuals who will be handling the human samples provided under the resulting contract. Detailed quality control and quality assurance plans will be required, an inventory system for tracking of samples during receipt, storage, processing, and shipping. Extractions of DNA from approximately 30,000 human or animal samples over five years are anticipated. Molecular analysis of approximately 50,000 human or animal samples over five years are anticipated. The offeror should be familiar with several methods of genotyping including TaqMan quantitative PCR, mass spectrometry, array hybridization, genetic bit analysis, primer extension, allele specific amplication, or other validated high-throughput methods. Offerors shall be required to perform a model study for proposal submission purposes. The estimated period of performance is five (5) years. The Government estimates that this five year period may require approximately 47,500 labor hours. This acquisition is a total small business set aside, the size standard for which is 500 employees. The Request for Proposal (RFP) will be available after January 8, 2001. See Numbered Notes 1 and 26.*****

Web Link

None (None)

Record

Loren Data Corp. 20001208/ASOL008.HTM (W-341 SN5082G8)