B -- CLONE, SEQUENCE AND ANALYZE RESULTS OF PCR PRODUCTS

Notice Date

March 7, 2001

Contracting Office

Department of Health and Human Services, Center for Disease Control and Prevention, Procurement and Grants Office (Atlanta), 2920 Brandywine Road, Room 3000, Atlanta, GA, 30341-4146

ZIP Code

30341-4146

Solicitation Number

2001Q00043

Response Due

March 23, 2001

Point of Contact

Susan Lyle, Purchasing Agent, Phone (770)488-2627, Fax (770)488-2670, Email SLyle@CDC.GOV -- Susan Lyle, Purchasing Agent, Phone (770)488-2627, Fax (770)488-2670, Email SLyle@CDC.GOV

E-Mail Address

Susan Lyle (SLyle@CDC.GOV)

Description

Delete the description reflected on solicitation 2001Q00043, March 6, 2001, and replace with the following: The Centers for Disease Control and Prevention (CDC), National Center for Infectious Diseases, Viral Exanthems and Herpesvirus Branch intends to procure the services of a vendor to clone, sequence, and analyze 16S rDNA and Mycoplasma spp amplicons produced subsequent to amplification of purified plasma DNA from CFS cases and controls. Minimum vendor qualifications: Ability to expand on current line of investigation and experimentation using plasma DNA samples from well-defined populations of CFS cases and controls. Knowledge and experience in handling and processing limited clinical material derived from patient and control plasma. Knowledge and experience in successfully identifying and characterizing previously unknown and/or novel bacterial pathogens using broad range PCR of 16S rDNA. Knowledge and experience in optimal conditions for amplification of bacterial and rickettsial pathogens from limited quantities of purified DNA from human plasma. Knowledge and experience in cloning and sequence analysis of the 16S rDNA products. Knowledge and experience for optimizing amplification conditions for genus-specific Mycoplasma primers. Knowledge and experience in cloning and sequence analysis of amplified mycoplasmal DNA. Knowledge and experience in interpreting the public health significance of findings All inquiries must reflect the RFQ number. Point of Contact is Sue Lyle at the address shown, Mailstop K70, or via e-mail at SIL2@cdc.gov, or by facsimile at 770-488-2670 or 2671. A Request for Quotations will be issued on approximately March 26, 2001. NAICS 611310, 621511, 622110.

Web Link

Visit this URL for the latest information about this (http://www.eps.gov/cgi-bin/WebObjects/EPS.woa/wa/SPF?A=M&P=2001Q00043&L=2965)

Record

Loren Data Corp. 20010309/BSOL004.HTM (D-066 SN50F4X5)