99 -- CELL BIOLOGIST (FLOW CYTOMETRIST) TO CULTURE AND MAINTAIN HEMOATOPIETIC, ERYTHROID AND NEOPLASTIC CELL

Notice Date

December 4, 2001

Contracting Office

National Institutes of Health, National Institute of Diabetes, Digestive and Kidney Diseases, Acquition Management Branch, 2 Democracy Plaza, Room 780, 6707 Democracy Blvd. MSC 5455, Bethesda, Maryland 20892-5455

ZIP Code

20892-5455

Solicitation Number

NIH-NIDDK-02-712

Response Due

January 8, 2002

Point of Contact

Patricia Haun, Purchasing Agent, 301-594-8855

Description

The National Institutes of Health (NIH), National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) has a requirement to purchase the services of a Cell Biologist (Flow Cytometrist) to Culture and Maintain Hemoatopietic, Erythroid and Neoplastic Cell for one year. The requirements for the person are as follows: 1) High speed cell sorting using a Coulter elite instrument. The candidate should be highly familiar with the instrument and be able to align lasers, repair minor hardware faults, and operate the software for data generation, retrieval, and statistical analysis. The candidate must possess expertise in high-speed sorting of human and other mammalian hematopietic cells. The candidate must have expertise in identifying erythroid cell and erythroid cell precursor populations among mixed populations of primary and neoplastic cells. 2) Staining and preparation of cells for flow cytometry. The candidate must possess expertise in staining and preparation of cellular molecules for flow cytometric detection. These include surface molecules, as well as, nucleic acids and lipid moieties. The candidate must be skilled in staining methods specifically applicable to erythroid cell subsets. 3) Culturing of primary human erythroid cells and mammalian cell lines. The candidate must be capable of media preparation and sterile techniques involved in the culture of primary human hematopoietic cells and neoplastic cells. Special expertise must include knowledge of erythroid cell culture methods. The candidate must also be aware of precautions used in the handling of samples infected with human pathogens. 4) Cell transfection with genetic materials and assessment of gene transfer efficiencies using flow cytometry. The candidate must be familiar with an able to perform gene transfer experiments using genes encoding green fluorescent protein. Expertise with flow-cytometric assessment of gene transfer is required. 5) Reagent storage and records. The candidate must be able to order and maintain the reagents necessary for the above requirements. The candidate must also be able to maintain the frozen laboratory cell stocks. All responsible small business sources and 8(a) sources may submit a quotation, which if timely received, shall be within the Simplified Acquisition Threshold for Purchases up to $100,000. It is the intent of the National Institute of Diabetes and Digestive Kidney Diseases to purchase the service of a Cell Biologist specified in the Request for Quotation (RFQ) NIH-NIDDK-02-712 will be available fifteen (15) days after the publication date of this synopsis. Receipt of the quotations will be due ten (10) days after the release of the RFQ. The anticipated award date is January 7, 2002. Request for copies must be in writing and may be FAXED to 301-480-4226. Quotations must be submitted to the National Institute of Diabetes and Digestive and Kidney Disease: 6707 Democracy Blvd., Suite 774C, Bethesda, Maryland 20817 Attention: Patricia Haun. FAX quotations will only be accepted if dated and signed by an authorized company representative.

Record

Loren Data Corp. 20011206/99SOL001.HTM (W-338 SN5143J6)